Environment-friendly composite mildew-proof preservative and application thereof

An environmentally friendly, antiseptic technology, applied in the direction of application, biocide, disinfectant, etc., can solve the problems of white sediment and turbidity, loss of the function of preserving specimens, and concentration reduction, so as to achieve no secondary pollution and scientific composition Safe and low toxicity effect

Inactive Publication Date: 2020-06-19
SHENZHEN INST OF GUANGDONG OCEAN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, specimens stored in formalin for a long time will harden, yellow, and have white precipitates and turbidity; the preservation solution is also prone to volatilization of formaldehyde, and the concentration decreases, gradually losing the function of preserving specimens, and needs to be replenished regularly or replace

Method used

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  • Environment-friendly composite mildew-proof preservative and application thereof
  • Environment-friendly composite mildew-proof preservative and application thereof
  • Environment-friendly composite mildew-proof preservative and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the preparation of environment-friendly composite anti-mildew preservative

[0045] The invention provides an environment-friendly composite anti-mildew preservative mainly composed of the following components: chitosan 100-10000PPM, chitosan quaternary ammonium salt 100-10000PPM, nano-silver 100-10000PPM, sodium benzoate 100-10000PPM, Kai Pine 100-10000PPM, the balance is water.

[0046] According to the preparation of the above components, an environment-friendly compound anti-mildew preservative can be obtained.

Embodiment 2

[0047] Example 2: Anti-mildew effect detection of environment-friendly compound anti-mildew preservative

[0048] 1) Selected strains for testing: Aspergillus niger, Aspergillus oryzae, Trichoderma, Penicillium, Aspergillus white, and Aspergillus flavus (all are molds isolated from biological specimens);

[0049] 2) Detection method: plate inhibition zone method (using nutrient agar plate);

[0050] 3) Detection temperature: 26℃±2℃;

[0051] 4) Detection time: 72 hours;

[0052] 5) test result: antiseptic preparation of the present invention all has obvious inhibitory effect to selected bacterial classification, and the diameter of inhibition zone is as follows: Aspergillus albicans 160mm, Aspergillus niger 130mm, Aspergillus flavus 136mm, Penicillium 130mm, Trichoderma 113mm, rice Aspergillus 142mm.

Embodiment 3

[0053] Embodiment 3: the bacteriostasis effect detection of environment-friendly compound anti-mildew preservative

[0054] 1) Selected test strains: Bacillus subtilis, Escherichia coli, Proteus, Streptococcus, Staphylococcus aureus, etc. (all are routine laboratory strains);

[0055] 2) Detection method: plate inhibition zone method (using nutrient agar plate);

[0056] 3) Detection temperature: 35°C±1°C;

[0057] 4) Detection time: 24 hours;

[0058] 5) Test result: measure with a caliper. Experimental results show that the antiseptic preparation of the present invention has obvious inhibitory effects on the selected strains, and the diameter of the inhibition zone is between 20-35mm.

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Abstract

The invention provides an environment-friendly composite mildew-proof preservative and an application thereof, and belongs to the technical field of marine organism mildew prevention and corrosion prevention. The environment-friendly composite mildew-proof preservative is mainly prepared from the following components in concentration content: 100 to 10,000 PPM of chitosan, 100 to 10,000 PPM of chitosan quaternary ammonium salt, 100 to 10,000 PPM of nano-silver, 100 to 10,000 PPM of sodium benzoate, 100 to 10,000 PPM of Kathon and the balance of water. The environment-friendly composite mildew-proof preservative is environment-friendly, odorless, colorless and low in toxicity, effectively reduces damage of volatile smell to human bodies, has little harm to the human bodies and the environment, is easy to manufacture and simple and convenient in method, has good preservation, fixation, sterilization, mildewproof and preservative effects on specimens, has great popularization value, and is an ideal substitute product of a formalin preservation liquid.

Description

technical field [0001] The invention relates to the technical field of anti-mildew and anti-corrosion of marine organisms, in particular to an environment-friendly composite anti-mold anti-corrosion agent and its application. Background technique [0002] There are many kinds of marine organisms, and the methods of making and preserving different types of biological specimens are also different. With the deepening of marine biological research, the collection and preservation of marine biological specimens is of great significance. At the same time, marine biological specimens, as an important material for biology, are an important model for teaching and an important carrier for scientific communication. Therefore, making marine organisms into specimens has important teaching and scientific significance. [0003] Specimens are easily damaged by mold hazards, resulting in shortened service life and economic losses. Therefore, anti-mold and anti-corrosion is a key part of spe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00A01N43/16A01N37/10A01N59/16A01N43/80A01P3/00A01P1/00
CPCA01N1/00A01N37/10A01N43/16A01N43/80A01N59/16
Inventor 张锐孙美榕刘羽赵志辉王润莲
Owner SHENZHEN INST OF GUANGDONG OCEAN UNIV
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