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Camellia oleifera seed meal antimicrobial polypeptide medical dressing and preparation method and application thereof

A technology of Camellia oleifera seed meal and antibacterial polypeptide, applied in medical science, absorbent pads, bandages, etc., can solve the problems of lack of microbial inhibition and removal ability such as bacteria, poor moisturizing function, unfavorable human skin, etc., and achieve stable antibacterial effect And moisturizing properties, good healing environment, good effect of antibacterial effect

Active Publication Date: 2020-06-19
黄山荷琇生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing dressings, gauze, etc., on the one hand, have poor moisturizing functions and cannot provide a good healing environment for the wound; on the other hand, they lack the ability to inhibit and remove bacteria and other microorganisms
In order to avoid the growth of bacteria and other microorganisms in the wound, metal antibacterial agents, antibiotics or other preservatives are usually added to inhibit the growth of microorganisms, but these antibacterial agents will cause poisoning or other sensitive phenomena to the human body, which is not good for human skin
In addition, since the wound may have exudate, if it is not cleaned in time, it will cause secondary damage to the wound

Method used

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  • Camellia oleifera seed meal antimicrobial polypeptide medical dressing and preparation method and application thereof
  • Camellia oleifera seed meal antimicrobial polypeptide medical dressing and preparation method and application thereof

Examples

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preparation example Construction

[0041] The preparation method of camellia oleifera seed meal antibacterial polypeptide medical dressing among the present invention comprises the following steps:

[0042] Step 1: Crush the camellia oleifera seed meal after oil extraction, add it into water, adjust the pH to 10.0-11.0, and stir it for a certain period of time, then centrifugally filter the supernatant, adjust the pH to 2.0-4.5 until the precipitate is produced, and centrifugally filter Get the precipitate, dissolve it in water again, adjust the pH to 2.0-4.0 and add porcine pepsin, stir at 37°C, raise the temperature to 90°C for 10 minutes to inactivate the enzyme, centrifuge to obtain the supernatant, adjust the pH to near Neutral, go through ultrafiltration membrane for centrifugal ultrafiltration, collect the filtrate with a molecular weight of less than 10kDa, freeze-dry, and obtain the antimicrobial peptide of camellia oleifera seed meal;

[0043] Step 2: Prepare a gelatin solution with a concentration of...

Embodiment 1

[0045]Step 1: Grind 40g of Camellia oleifera seed meal after oil extraction to 60 mesh, add 600mL water to adjust the pH to 10.3 with 0.5mol / L sodium hydroxide solution, stir well for 2 hours, then centrifugally filter to take the supernatant, and use 0.5mol / L sodium hydroxide solution to adjust the pH to 10.3. mol / L hydrochloric acid solution to adjust the pH to 2.5 to produce precipitates, centrifuge to obtain precipitates, re-dissolve in 20mL water, adjust the pH to 3.7 (adjust with 0.5mol / L hydrochloric acid solution) and add pig-derived pepsin (according to its reaction Add 0.5% of the substrate mass), stir at 37°C for 1.5h, raise the temperature to 90°C for 10min to inactivate the enzyme, centrifuge to obtain the supernatant, adjust the pH to near neutral with 0.1mol / L sodium hydroxide , carry out centrifugal ultrafiltration through an ultrafiltration membrane (an ultrafiltration membrane with a molecular weight cut-off of 10kDa), collect the filtrate with a molecular wei...

Embodiment 2

[0048] Step 1: Grind 40g of Camellia oleifera seed meal after oil extraction to 60 mesh, add 0.5mol / L sodium hydroxide solution to 800mL water to adjust the pH to 10.5, stir well for 2 hours, then centrifugally filter to take the supernatant, and use 0.5mol / L sodium hydroxide solution to adjust the pH to 10.5. / L hydrochloric acid solution to adjust the pH to 3.0 to produce a precipitate, centrifuge to obtain the precipitate, dissolve it in 20mL of water again, adjust the pH to 3.0 (regulate with 0.5mol / L hydrochloric acid solution) and add pig-derived pepsin (according to its reaction base Add 1.0% of the amount of substance), after stirring at 37°C for 1h, raise the temperature to 90°C for 10min to inactivate the enzyme, centrifuge to obtain the supernatant, adjust the pH to nearly neutral with 0.1mol / L sodium hydroxide, and The ultrafiltration membrane is subjected to centrifugal ultrafiltration, and the filtrate with a molecular weight of less than 10kDa is collected and fr...

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Abstract

The present invention discloses a camellia oleifera seed meal antimicrobial polypeptide medical dressing and a preparation method and an application thereof. The method comprises the following steps:(1) crushing camellia oleifera seed meal after oil pressing, adding the crushed camellia oleifera seed meal to water, adjusting pH value to 10.0-11.0, and conducting stirring and centrifugation to obtain a supernatant; then adjusting pH value of the supernatant to be 2.0-4.5 to produce a precipitate; then taking the precipitate, dissolving the precipitate in water, adjusting pH to 2.0-4.0, addingprotease for enzymatic hydrolysis reaction, taking a supernatant after enzymatic hydrolysis, adjusting pH value to be neutral, and conducting ultrafiltration and freeze-drying to obtain camellia oleifera seed meal antimicrobial polypeptide; and (2) dissolving the camellia oleifera seed meal antimicrobial peptide in water, then adding a gelatin solution and a sodium alginate solution, and conducting freeze-drying to obtain the camellia oleifera seed meal antimicrobial polypeptide medical dressing. The medical dressing obtained by the method has a good antimicrobial effect and a good moisturizing performance, and can be applied in the fields of wound protection, wound repair. etc.

Description

technical field [0001] The invention relates to the fields of medical wound protection and wound repair, in particular to a camellia oleifera seed dregs antibacterial polypeptide medical dressing and its preparation method and application. Background technique [0002] Dressing is a clinical medical material used to cover wounds, which can isolate the wound from direct contact with the external environment. Existing dressings, gauze, etc., on the one hand, have poor moisturizing functions and cannot provide a good healing environment for wounds; on the other hand, they lack the ability to inhibit and remove bacteria and other microorganisms. In order to avoid the growth of bacteria and other microorganisms in the wound, metal antibacterial agents, antibiotics or other preservatives are usually added to inhibit the growth of microorganisms, but these antibacterial agents will cause poisoning or other sensitive phenomena to the human body, which is not good for human skin. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/46A61L15/42
CPCA61L15/32A61L15/46A61L15/42A61L2300/252A61L2300/404C08L89/00
Inventor 李成威康清俊鲍时信程建华陈鸿毅
Owner 黄山荷琇生物科技有限公司
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