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A kind of preparation technology of brain polypeptide

A technology of brain polypeptide and sheep brain, which is applied in the field of preparation technology of brain polypeptide, can solve the problems of insufficient health care effect, achieve the effect of easy health care effect, improve health care function, and increase the hydrolysis rate

Active Publication Date: 2021-08-13
江西康宝医药生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Sheep brain protein is rich in content and high in acidic amino acid content, which is an excellent resource for developing antioxidant active peptides. In the prior art, some people use protease to hydrolyze sheep brain protein to produce antioxidant polypeptides, but its health care effect is insufficient.

Method used

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  • A kind of preparation technology of brain polypeptide
  • A kind of preparation technology of brain polypeptide
  • A kind of preparation technology of brain polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation process of brain polypeptide, the steps are as follows:

[0036] (1) Sheep brain degreasing pretreatment:

[0037] Collect fresh sheep brain, wash and remove impurities and other pretreatments, pre-freeze and freeze-dry for 48 hours to freeze-dry, freeze-dried sheep brain is crushed, passed through a 100-mesh sieve, extracted and degreased by petroleum ether Soxhlet for 12 hours, and then placed in a fume hood For 12 hours, volatilize the remaining petroleum ether and store at -60°C;

[0038] (2) Strain activation:

[0039] Lactobacillus plantarum No. 3: inoculate Lactobacillus plantarum No. 3 into MRS solid medium to streak, culture anaerobically at 37°C for 24 hours, pick a single colony and inoculate it into MRS liquid medium for anaerobic culture to 0.5*10 7 cfu / ml Lactobacillus plantarum No. 3 seed liquid;

[0040] Activation of B. subtilis sp.nk1: use nutrient broth medium (1L distilled water + 20g glucose + 15g peptone + 5g sodium chloride + 0.5g...

Embodiment 3

[0060] Embodiment 3: Determination of hydrolysis rate

[0061] The main detection method that the present invention uses is:

[0062] Determination of total protein content: trace Kjeldahl method GB / T5009.5-2003

[0063] Determination of Soluble Peptide Content

[0064] Folin-phenol method: prepare Folinol A, B solutions and a series of bovine serum albumin (BSA) standard solutions with different concentration gradients, use water as a reference, measure the absorbance at 640 nm, and draw a standard curve. Dilute the sheep cerebroprotein fermentation broth to an appropriate multiple, pipette 1 mL and follow the steps of making a standard curve, measure the absorbance at 640 nm, and obtain the corresponding BSA content from the regression equation, so as to obtain the content of soluble peptides.

[0065] Determination of degree of hydrolysis:

[0066] Degree of hydrolysis (DH)%=(N2-N1) / (N0-N1)

[0067] N0 is the total protein in sheep cerebroprotein, N1 is the TCA soluble ...

Embodiment 4

[0071] Determination of Absorbability of Brain Polypeptides Through the Intestines

[0072] The cerebropeptide solutions of Example 1, Comparative Example 1 and Comparative Example 2 were centrifuged at 4000 r / min for 20 min after enzyme sterilization, and the supernatant was collected, filtered through filter paper, concentrated in vacuum to an appropriate amount, and then freeze-dried. Brain peptide products can be obtained.

[0073] The guinea pigs were killed quickly and the ileum was removed, the mesentery was removed, washed with warm maintenance solution, a section of about 5 cm was cut, one end was ligated, the mucosa was facing outward, the liquid on the surface of the mucosa was blotted with filter paper, and the flat-tip injection needle was never ligated with a micro-syringe Inject an appropriate amount of maintenance solution into the intestinal sac from the end to the extent that the villi on the surface of the mucosa are straightened, and tie it. Put it into a ...

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Abstract

The invention discloses a brain polypeptide preparation process: (1) degreasing pretreatment of sheep brain; (2) strain activation; (3) anaerobic fermentation of Lactobacillus plantarum No. 3; (4) Bacillus subtilis sp. .nk1 aerobic fermentation; (5) high temperature inactivation.

Description

technical field [0001] The invention relates to a preparation process of a brain polypeptide, which belongs to the field of biotechnology, in particular to a preparation process of a brain polypeptide, especially a preparation process of a brain polypeptide using probiotic fermentation technology. Background technique [0002] Cerebral protein hydrolyzate refers to a complex system composed of abundant free amino acids and polypeptides with a molecular weight of less than 10,000 produced by hydrolyzing degreased animal brain marrow with protease. Animal protein hydrolyzate is mainly used in the treatment of cerebrovascular disease as a specific drug for cerebrovascular disease. At present, the most widely used is pig brain protein hydrolyzate obtained by hydrolyzing healthy pig brain with protease after degreasing. However, the research on other animal brain proteins is not in-depth. Some researchers have carried out enzymatic research on chicken brain, cow brain, sheep brai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
CPCA23L33/18A23L29/065A23V2002/00A23V2400/169A23V2200/30A23V2250/55
Inventor 聂建群
Owner 江西康宝医药生物科技有限公司
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