Centrifugal high-throughput droplet preparation chip

Abstract

The invention discloses a centrifugal high-throughput droplet preparation chip, which includes a chip body, a clamping structure and one or more high-throughput droplet preparation units arranged on the chip body; the high-throughput The droplet preparation unit includes a sample cavity, a buffer cavity, a droplet collection cavity and a waste liquid cavity connected in sequence; the buffer cavity and the droplet collection cavity are communicated through a microhole array. The invention provides a centrifugal high-throughput microdroplet preparation chip, which can realize the preparation of high-throughput microdroplets by utilizing the characteristics that each hole in the microwell array is simultaneously prepared in parallel under the drive of centrifugal force, and the preparation rate can reach 50 to 100 times of the existing technology; while maintaining the advantages of simplicity, easy implementation and high consistency. The centrifugal high-throughput micro-droplet preparation chip of the present invention has positive significance for improving the micro-droplet preparation technology and its application in technical fields such as micro-droplet digital PCR.

Description

technical field [0001] The invention relates to the field of microfluidics, in particular to a centrifugal high-throughput microdroplet preparation chip. Background technique [0002] Droplet-based microfluidics (droplet-based microfluidics) technology is a technology that uses the fluid shear force of the continuous phase to destroy the surface tension of the discrete phase at the microscale, and divides the discrete phase into nanoliter or even picoliter droplets. Microdroplets prepared based on microfluidic technology have the advantages of small size, high precision, and complete isolation between droplets. It is a very excellent microreactor and has been widely used in mass spectrometry, gene screening, protein synthesis, and digital PCR. and other fields. [0003] At present, the microdroplet preparation method is based on the "T" channel method (T Junction) (see attached figure 1 ①), Flow Focusing (see appendix figure 1 ②), coaxial method (Co-Flow) (see attached f...

AI Technical Summary

Problems solved by technology

However, the complexity of this parallel preparation technology increases with the increase in the number of parallel microstructures, the complexity of its flow path system increases sharply, and the manufacturing cost is relatively high.

At the same time, the processing difference of each flow channel also reduces the size consistency of the prepared droplets

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