Detection agent composition and kit for detecting multiple mutation sites of EGFR gene

Abstract

The invention provides a detection agent composition and kit for detecting multiple mutation sites of an EGFR gene. The invention firstly provides the detection agent composition for detecting the multiple mutation sites of 18-21 exons of the EGFR gene. The detection agent composition contains SEQ ID No.1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, SEQ ID No.6, SEQ ID No.7, SEQ ID No.8, SEQ ID No.9, SEQ ID No.10 and SEQ ID No.11. The invention further provides the relevant detection kit and a detection method. The detection agent composition and the detection kit are capable of simultaneously detecting 51 mutations of the EGFR gene, the detection rates of the mutations are basically the same, the detection sensitivity can reach up to 0.1%, and meanwhile, the detection agent composition and the detection kit are applicable to mutation detection in cfDNA and tissue DNA.

Description

technical field [0001] The invention relates to a primer, a probe, a multiple qPCR reaction system and a kit for simultaneously detecting 51 kinds of mutations on exons 18-21 of the human EGFR gene, belonging to the fields of biotechnology and medicine. Background technique [0002] EGFR is the expression product of the proto-oncogene c-erbB1 and a member of the epidermal growth factor receptor (HER) family. The HER family consists of four molecules, EGFR / HER1 / erbB1, HER2 / neu / erbB2, HER3 / erbB3, and HER4 / erbB4, which play important regulatory roles in physiological processes such as cell growth, proliferation, and differentiation. [0003] EGFR is a transmembrane tyrosine kinase receptor, and the activation of the receptor kinase domain is related to various signal transduction pathways such as cancer cell proliferation, metastasis and apoptosis. Abnormalities in EGFR signaling are responsible for the development of various tumors. [0004] EGFR mutations mainly occur in th...

AI Technical Summary

Problems solved by technology

However, the current digital PCR instrument can only detect the signals of two channels at the same time, and cannot achieve more PCR detection. The throughput is low, the detection process is cumbersome, time-consuming, and costly.

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