Method for cultivating diplars of the common octopus octopus
A larval, octopus technology, applied in fish farming, application, climate change adaptation and other directions, can solve problems such as insufficient results, and achieve the effect of reducing impact and increasing profitability
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Embodiment 1
[0061] Example 1: Experiments on culture of octopus larvae known in the prior art (Garrido, D. et al. (2017) .Aquaculture, 468, 558-568):
[0062] The experiments were carried out in a truncated conical tank with a volume of 500 L, the walls and bottom of which were black, with light to moderate aeration in the center. Green water (phytoplankton) was used and renewed 150% daily throughout the experimental period (30 days), and the water was filtered at 2 microns by means of a cartridge filter. As far as the light is concerned, 36W cool white fluorescent lamps are used, which are located in the upper central part of the pool. A single intensity of 700 lux was used throughout the experimental period. The food is based on a species of Artemia that is rich in microalgae and another rich in marine phospholipids. Artemia species were supplied in 3 doses throughout the day. Survival rates at 30 days of age ranged from 0.14 to 3.77%, while growth rates showed dry weight increas...
Embodiment 2
[0063] Embodiment 2: control experiment: common octopus larvae are cultivated (1000l pond), from the 1st day to the 1st day of the life of dilarvae For 40 days only Artemia species were used as bait.
[0064] The experiment was carried out in a truncated conical pool with a volume of 1000l, the walls and bottom were black, and the central position was moderately ventilated to prevent the water flow produced by the air bubbles from hindering the diplars from freely passing through the pool ( Figure 5A ). For a temperature range of 18.5 to 21.3° C. and a salinity of 35 g / l (over the duration of the experiment), the density was 5 diplopia / l and the values of dissolved oxygen fluctuated between 5.5 and 6.7 mg / l. Use green water for up to 30 days, with 10 when adding microalgae 6 Nannochloropsis spp. and Isochrysis aff. galbana at cls / mL concentrations. The method is the same as the previous experiment (the prior art experiment), but in this second experiment a newer modif...
Embodiment 3
[0067] Example 3: Common octopus larvae culture test (1000 l pool), using Artemia species and Crab fleas and amphipods treated with the present invention from day 31 onwards.
[0068] This experiment was carried out in parallel with the previous experiment, using the same culture conditions, with the aim of comparing the effect of different baits. In this case, Artemia species were supplemented with crab fleas (0.1 / ml) until 30 days of age (however, due to difficulties in obtaining fleas, these could only be added to the ponds for 10 of the first 30 days, which reduced expected growth). From then on, only amphipods were provided for them. On the one hand, the species of the suborder of the genus Glycerus (preferably the genus Sarcophagus and the genus Glycophyllum) accounting for 95% of the total number of individuals, and the species of the genus Phtisica and / or the stalk insects of the species of the genus Caprella (preferably Phtisica marina and long-branched straw wo...
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