Separation method of soil microorganisms capable of inhibiting wheat ear rot pathogenic microorganisms

Abstract

The present invention discloses a separation method of soil microorganisms capable of inhibiting wheat ear rot pathogenic microorganisms. A soil extract diluted to 10<-2> times, 10<-3> times, and 10<-4> times is prepared in advance, barley grains are also used for culturing wheat ear rot pathogenic microorganisms, and then an inhibitory effect of microorganisms in the soil extract on the wheat earrot pathogenic microorganisms infecting the barley grains is utilized for effective screening. Since many ear rot pathogenic microorganisms exist on the infected barley grains and the number of the microorganisms in the spray diluted soil extract is extremely small, an absolute difference in the number exist. Therefore, microorganism colonies that can survive among the ear rot pathogenic microorganisms should be the colonies with strong fecundity and anti-microorganism effect, thus ineffective microorganisms can be effectively eliminated, a purpose is clear, workload of blindly separating thesoil microorganisms can be greatly reduced, labor and cost are saved, effective microorganisms are not liable to miss, separation efficiency of the soil microorganisms capable of inhibiting the wheatear rot pathogenic microorganisms is improved and the separation method is high in practicality.

Description

technical field [0001] The invention relates to the technical field of microbiology, in particular to a method for isolating soil microorganisms that inhibit wheat ear rot pathogens. Background technique [0002] Common ear rot pathogens that damage wheat, barley, corn, rice and other gramineous plants include Gibberella zeae, Fusarium gramineae in the asexual stage, Helminthes sp. The fungus uses mycelium to overwinter or oversummer in diseased residues such as rice piles or seeds. In the next growing season, it forms ascus shells and emits ascospores, which are transmitted by airflow for initial infection, and conidia are transmitted by rainwater for re-infection. High temperature, rainy, foggy and humid weather are conducive to the occurrence of this disease. [0003] The control methods of wheat ear rot pathogen include physical control, chemical fungicide control, selection of disease-resistant wheat varieties and biological control. Among them, biological control is ...

AI Technical Summary

Problems solved by technology

But soil is the base camp of microbial life, and there are as many as 10 microorganisms in 1g of soil. 6 ~10 9 For individual microorganisms, if the soil microorganisms are separated first and then the antibacterial test is carried out, it is easy to cause a huge workload of soil separation, and it is easy to miss the effective microorganisms with inhibitory effect.

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