In-situ enriching method and screening method of pullulanase indigenous strains
An in situ enrichment, pullulanase technology, applied in biochemical equipment and methods, determination/inspection of bacteria, microorganisms, etc. Easy to operate, clear purpose, reliable effect
Active Publication Date: 2018-01-05
HENAN UNIV OF SCI & TECH
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Abstract
The invention relates to an in-situ enriching method and a screening method of pullulanase indigenous strains. The in-situ enriching method comprises the following steps: burying a matrix to which amylopectin is adhered into soil; enriching; then taking out the matrix; and collecting soil on the surface of the matrix to obtain the strain-enriched soil. The screening method comprises the followingsteps: uniformly mixing the strain-enriched soil and sterile water; centrifuging; performing gradient dilution on an upper suspension; coating a flat separating plate with the gradient diluting solution; culturing; and then identifying. The enriching method has the major characteristic that amylopectin is used as a primary inducing substrate for in-situ enriching the pullulanase indigenous strainsin soil, so that the quantity of pullulanase indigenous populations can be increased in an original ecological environment, and particularly some weak populations with pullulanase activity can be certainly proliferated, and as a result, the detection rate of the target strains can be increased.
Application Domain
BacteriaMicrobiological testing/measurement +1
Technology Topic
PopulationPullulanase activity +7
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