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Method for determining melatonin in faeces by using high performance liquid chromatography

A technology of high-performance liquid chromatography and melatonin, which is applied in the field of high-performance liquid chromatography for the determination of melatonin in feces, can solve the problems of complex animal feces components, human, material and economic losses, and affect production performance, and achieve high sensitivity, The results are accurate, reliable and stable

Pending Publication Date: 2020-08-07
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Obtaining animal tissue, plasma, and serum will cause certain damage to the animal body, affect its production performance, and also require a lot of manpower and material resources to cause economic losses
It is convenient to collect animal feces to detect melatonin, which can avoid trauma to animals and reduce economic losses. The composition of animal feces is complex, and pretreatment of feces is a difficult point when detecting melatonin in animal feces. However, no animal feces have been seen yet. Pretreatment and report on the detection method of melatonin in animal feces

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  • Method for determining melatonin in faeces by using high performance liquid chromatography
  • Method for determining melatonin in faeces by using high performance liquid chromatography
  • Method for determining melatonin in faeces by using high performance liquid chromatography

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Embodiment Construction

[0015] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. This invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete and will fully convey the concept of the invention to those skilled in the art, and the present invention will only be defined by the appended claims.

[0016] 1. Chromatographic conditions

[0017] Chromatographic column: C18 chromatographic column 250×4.6mm, 5um; column temperature: 25°C; detector: fluorescence detector; excitation wave Ex: 285nm, emission wave Em: 345nm; flow rate: 0.5mL / min, injection volume: 20uL .

[0018] 2. Preparation of reference solution and drawing of standard curve

[0019] Take a certain amount of melatonin standard subs...

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Abstract

The invention discloses a method for determining melatonin in faeces by using high performance liquid chromatography. The method comprises the following steps: mashing and uniformly mixing to-be-determined faeces, freeze-drying the to-be-determined faeces by using a freeze dryer, leaching the to-be-determined faeces by using methanol, centrifuging, taking a supernatant, and blow-drying the supernatant by using a termovap sample concentrator; performing dissolution by using an acetonitrile n-hexane solution, centrifuging, taking a subnatant, and filtering the subnatant by using a microfiltration membrane; and performing high performance liquid chromatography detection on a machine, and quantifying the melatonin in the faeces sample by adopting an external standard method. The melatonin in the faeces is extracted and detected for the first time; the detection method is high in sensitivity, high in specificity, good in stability and accurate and reliable in result; a novel animal non-invasive melatonin detection method can be established; and the circadian rhythm of animal endogenous melatonin secretion is clarified.

Description

technical field [0001] The invention relates to the technical field of melatonin detection, in particular to a method for measuring melatonin in feces by using high performance liquid chromatography. Background technique [0002] Melatonin (Melatonin, MT) is an indole hormone, chemical name is N-acetyl-5-methoxytryptamine, and it is a broad-spectrum physiological regulator. Melatonin mainly comes from the pineal gland of mammals. It has been proven to be the endogenous free radical scavenger with the strongest antioxidant effect known so far. It plays an important role in sleep, promotion of gonad development and circadian rhythm. Animals have discovered the existence of melatonin, which can regulate circadian rhythm, photoperiod and growth and development. The functions of melatonin in animals include scavenging free radicals, enhancing immunity, and inhibiting aging. Its physiological and pharmacological functions The diversity of melatonin has been generally valued by sc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/047G01N2030/146
Inventor 熊家军李可可何长久齐德生陈建国许学林荆焕松孙旭阳
Owner HUAZHONG AGRI UNIV
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