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Application of stenotrophomonas rhizophila in improvement of rhizosphere soil and promotion of plant growth

A technology for promoting plant growth and oligotrophic monocytogenes, applied in the application, soil conditioning materials, fertilizer mixtures, etc., can solve the problems of low utilization rate, low utilization rate of nitrogen fertilizer, large application amount, etc. The content of nitrogen and phosphorus and other elements, and the effect of improving physical and chemical properties

Inactive Publication Date: 2020-08-14
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is the largest producer and consumer of nitrogen fertilizer in the world, but the utilization rate of nitrogen fertilizer is low; the phenomenon of large application amount and low utilization rate of phosphorus fertilizer is also common in agricultural production

Method used

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  • Application of stenotrophomonas rhizophila in improvement of rhizosphere soil and promotion of plant growth
  • Application of stenotrophomonas rhizophila in improvement of rhizosphere soil and promotion of plant growth
  • Application of stenotrophomonas rhizophila in improvement of rhizosphere soil and promotion of plant growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The addition of Stenotrophomonas rhizophilus in the pot experiment of embodiment 1

[0046] (1) Preparation of Stenotrophomonas rhizophilus bacteria suspension

[0047] Pick the frozen glycerol strains, streak on the LB solid medium, incubate the plate upside down at 30°C for 48 hours, and wait until a single colony grows. Pick a single colony into LB liquid medium, 30°C, 150rpm, until the number of bacteria in the medium reaches 2.0×10 8 CFUml -1 , centrifuge and wash the bacterial solution twice with sterile water, remove the medium components, prepare a bacterial suspension, and store it at 4°C for short-term storage.

[0048] (2) Disinfection of plant seeds

[0049] Put the rapeseed seeds in 2% sodium hypochlorite (NaClO) solution and shake for 10 min, wash them with sterile water for 3 times, then place the seeds at 4°C, and after vernalization in the dark for 48 h, put the seeds into the light incubator (20 ℃, 12h / 12h day / night) for 7 days.

[0050] (3) Exper...

Embodiment 2

[0053] Embodiment 2 Stenotrophomonas rhizophilus is for the improvement of rhizosphere soil

[0054] 1. Method

[0055] (1) General soil sample collection

[0056] The soil (1-10 cm underground) area of ​​each potted plant was sampled, and the collected soil was mixed and defined as a common soil sample after using the five-point sampling method.

[0057] (2) Rhizosphere soil sample collection

[0058] Collect 10 plants in each pot, shake off the loose soil attached to the roots of the plants, cut off the roots, rinse thoroughly with sterile PBS buffer three times, collect all the washings and pass through a 0.22 μm filter membrane, filter and attach The soil on the film is defined as the rhizosphere soil, and the sampling diagram is shown in figure 2 .

[0059] (3) Testing of physical and chemical properties of soil

[0060] The physical and chemical properties of common soil and rhizosphere soil in Sr group and CK group were detected, including soil pH, total nitrogen ...

Embodiment 3

[0070] Effect of embodiment 3 Stenotrophomonas rhizophilus on plant growth

[0071] 1. Method

[0072] (1) Collection of plant samples

[0073] 10 plants were collected from each pot, that is, a total of 30 plants were collected from each treatment group, placed on ice and transferred to the laboratory.

[0074] (2) Detection of physical and chemical properties of plants

[0075] The contents of chlorophyll, soluble starch and soluble protein in the youngest fully open leaves of the plants were measured; the rest of the aboveground tissues of the plants were used to measure the biomass and total nitrogen (TN) content.

[0076] Determination of plant chlorophyll (Chlorophyll): measured using a chlorophyll meter (SPAD-502 plus, KONICAMINOLTA, Japan).

[0077] Determination of plant soluble starch (Soluble starch): Anthrone method.

[0078] Determination of plant soluble protein (Soluble protein): Coomassie brilliant blue method.

[0079] Determination of plant biomass (Biom...

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Abstract

One or more embodiments of the present specification provide an application of stenotrophomonas rhizophila in improvement of rhizosphere soil and promotion of plant growth. One or more embodiments ofthe invention further provide a method for improving rhizosphere soil and promoting plant growth. Stenotrophomonas rhizophila is added into the soil at the early stage of plant growth. The stenotrophomonas rhizophila can increase the content of nitrate nitrogen, phosphorus and other elements in rhizosphere soil, improve the physicochemical properties of the rhizosphere soil and increase the content of nitrogen in plants. Therefore, the stenotrophomonas rhizophila can replace chemical fertilizer to play a role in agricultural production.

Description

technical field [0001] One or more embodiments of this description relate to the field of biotechnology, and in particular to the application of Stenotrophomonas rhizophilus in improving rhizosphere soil and promoting plant growth. Background technique [0002] The growth of plants depends on the absorption of various nutrients from the soil, and the main elements that limit plant growth are nitrogen (N) and phosphorus (P). Nitrogen and phosphorus are one of the important elements in plants. They are important components of protein and nucleic acid, and have a direct impact on the growth and yield of plants. my country is the world's largest producer and consumer of nitrogen fertilizer, but the utilization rate of nitrogen fertilizer is low; the phenomenon of large application amount and low utilization rate of phosphorus fertilizer is also common in agricultural production. Contents of the invention [0003] In view of this, the purpose of one or more embodiments of this...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C05G3/80C09K17/14A01B79/02C09K101/00
CPCC05F11/08C05G3/80C09K17/14A01B79/02C09K2101/00
Inventor 庄绪亮刘颖高婕白志辉吴尚华
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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