Targeted growth factor for promoting scar endometrium reconstruction of infertile women and application of targeted growth factor

A growth factor, endometrium technology, applied in the biological field

Active Publication Date: 2020-08-28
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, there are few effective treatment...

Method used

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  • Targeted growth factor for promoting scar endometrium reconstruction of infertile women and application of targeted growth factor
  • Targeted growth factor for promoting scar endometrium reconstruction of infertile women and application of targeted growth factor
  • Targeted growth factor for promoting scar endometrium reconstruction of infertile women and application of targeted growth factor

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Experimental program
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Effect test

Embodiment 1

[0103] Embodiment 1, preparation of basic fibroblast growth factor (CBD-bFGF) with collagen binding ability

[0104] Basic fibroblast growth factor (CBD-bFGF) with collagen binding ability provided by the present invention is a protein formed by fusion of basic fibroblast growth factor (bFGF) and collagen binding region (CBD), and its amino acid sequence is as follows: It is shown in SEQ ID No.1 in the list, and its coding sequence is shown in SEQ ID No.2 in the sequence listing.

[0105] Wherein, the 25th-178th of SEQ ID No.1 is the amino acid sequence of basic fibroblast growth factor, and the 73rd-534th of SEQ ID No.2 is the coding gene sequence of basic fibroblast growth factor; SEQ ID No. 3-9 of No. 1 is the amino acid sequence of the collagen-binding region CBD, and No. 7-27 of SEQ ID No. 2 is the coding gene sequence of the collagen-binding region CBD.

[0106] 1. Preparation of recombinant vector

[0107] The small fragment between the NcoI recognition sequence (CCAT...

Embodiment 2

[0118] Embodiment 2, the clinical application of CBD-bFGF

[0119] 1. Subjects

[0120] The inclusion criteria of the experimental subjects of the present invention are as follows: infertile women aged 20-40, with a clear desire for fertility, with normal ovarian function or frozen embryos, willing to follow-up, diagnosed with endometrial scar and ineffective for drug and surgical intervention . Patients were excluded if they had any of the following: significant adhesions that could separate; abnormal karyotype; concomitant severe uterine disease, including large intramural fibroids, severe adenomyosis, severe Congenital uterine malformations, endometrial tuberculosis; hypertension, diabetes and other systemic diseases; contraindications to pregnancy; contraindications to hormone replacement therapy (HRT); history of pelvic tumors or pelvic radiotherapy; participation in other clinical studies .

[0121] The research object of the present invention is as follows: 20 infert...

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Abstract

The invention discloses a targeted growth factor for promoting scar endometrium reconstruction of infertile women and an application of the targeted growth factor. The collagen-targeted basic fibroblast growth factor is a protein formed by fusing a basic fibroblast growth factor and a collagen binding domain. Experiments prove that after collagen-targeted basic fibroblast growth factor treatment,the menstrual blood volume, the endometrium thickness and the scar endometrium area of each infertility patient are all improved. Histological study shows that the blood vessel density is obviously increased. Three patients keep pregnancy 20 weeks after pregnancy. One patient gives birth to a healthy baby. The collagen-targeted basic fibroblast growth factor is injected into the endometrium in a multi-point mode, so that the angiogenesis capacity and proliferation capacity of the endometrium are improved, and the persistent pregnancy rate is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a targeted growth factor for promoting scar endometrium reconstruction in infertile women and an application thereof. Background technique [0002] Endometrial adhesions (IUAs), also known as Asherman syndrome (AS), are the result of traumatic or inflammatory infestation of the endometrium and are a major cause of uterine infertility. For symptomatic IUAs, hysteroscopic adhesiolysis to restore normal anatomy of the uterine cavity is currently the only recommended approach. However, recurrence of IUAs is very common and difficult to treat, especially with severe adhesions. The endometrium is a unique cyclic tissue regeneration system that relies on the periodic growth and regression of endometrial blood vessels. Severe damage to the endometrium, that is, to the subepithelial capillary plexus, leads to blockage of endometrial regeneration. Natural cells, including epithe...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A61K38/18A61K47/64A61P15/08
CPCC07K14/503A61P15/08C07K2319/00A61K38/00
Inventor 戴建武胡娅莉陈冰侯祥林
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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