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A neutralizing antibody against n-protein of novel coronavirus

A coronavirus, a new type of technology, used in antiviral immunoglobulins, antiviral agents, antibodies, etc.

Active Publication Date: 2021-11-16
BEIBU GULF UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no specific vaccines for prevention and specific drugs for the treatment of new coronaviruses. Generally, non-specific treatments are used to prevent serious complications, reduce severe morbidity and mortality, and increase the cure rate.

Method used

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  • A neutralizing antibody against n-protein of novel coronavirus
  • A neutralizing antibody against n-protein of novel coronavirus
  • A neutralizing antibody against n-protein of novel coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Expression and purification of antibodies in FAB 20

[0016] 1.1 Looking for sequences of Fab 20 antibodies

[0017] The amino acid sequence of the Fab 20 antibody of the SARS-COV-2N antigen protein was determined from the antibody library of SARS-COV. Among them, the amino acid sequence of the amino acid sequence of SARS-COV-2N protein, the light chain of the Fab 20 antibody variable region, the amino acid sequence of the heavy chain (H CHAIN), and the full length of the Fab 20 antibody light chain The full length amino acid sequence of the amino acid sequence and the heavy chain is as follows:

[0018] The amino acid sequence of the SARS-COV-2N protein is:

[0019] MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAP...

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Abstract

The invention discloses a neutralizing antibody against the N protein of novel coronavirus, which consists of a light chain and a heavy chain, the amino acid sequence of the variable region of the light chain is shown in SEQ ID NO: 1, and the heavy chain can be The amino acid sequence of the variable region is shown in SEQ ID NO:2. The antibody of the present invention can specifically recognize and bind to the SARS-CoV-2 N protein. Experimental results show that its affinity constant KD value with 2019-NCOV Nucleocapsid is 111nM, and its EC50 value is 3.975 μg / mL; The EC50 value of SARI‑N‑his was 4.752 μg / mL.

Description

Technical field [0001] The present invention relates to a neutralizing antibody from an RNA virus, in particular to a neutralizing antibody against a new coronavirus N protein. Background technique [0002] The pathogens of new coronaviruses in 2019 are new coronaviruses (2019-NCOV, COVID-19 or SARS-COV-2). SARS-COV-2 belt Coronavirus, a class of single stranded RNA viruses having an envelope. The new coronavirus gene encodes a plurality of structural proteins, such as N protein, M protein, E protein, and S proteins, S proteins can be specific to the host receptor, which is a key protein, M protein of viral intrusion. And the formation of the E protein reference viral cell membrane, and N protein is involved in the assembly of the virus. These proteins include a plurality of antigenic epitopes, using the principles of antigens and antibody-specific binding, can directly prove the sample containing new coronaviruses in the sample. [0003] The new coronavirus spreads quickly and h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10A61K39/42A61P31/14G01N33/569G01N33/68
CPCA61K2039/505A61P31/14C07K16/10C07K2317/56C07K2317/92G01N33/56983G01N33/6854G01N2333/165
Inventor 吴信忠蔡小辉陈泓霖
Owner BEIBU GULF UNIV