Nucleic acid detection method and nucleic acid detection kit based on detection test paper display

Abstract

The invention discloses a nucleic acid detection method and nucleic acid detection kit based on the detection test paper display. The method comprises the following steps: providing a nucleic acid probe, wherein one end of the nucleic acid probe is connected with a solid phase particle by a covalent bond, and the other end of the nucleic acid probe is connected with an antibody recognition substance by a covalent bond; adding an amplified sample to be tested and the nucleic acid probe into a reaction system composed of crRNA and Cas protein, and carrying out a reaction to obtain a reaction solution; dropwise adding the reaction solution to a sample addition area of a detection test paper, wherein the sample addition area contains a colloidal gold-labeled first antibody, the detection testpaper comprises a second antibody site that specifically binds to the first antibody, and a streptavidin site that binds to the antibody recognition substance; and judging whether the sample to be tested contains target nucleic acid by observing a colored band of the detection test paper. The method can effectively avoid the weak positive and false positive problems in the previous nucleic acid detection, and thereby effectively improves the sensitivity and accuracy of a nucleic acid detection method based on the coloring of a detection test paper.

Description

technical field [0001] The invention relates to the field of nucleic acid detection, in particular to a nucleic acid detection method and a nucleic acid detection kit based on detection paper color development. Background technique [0002] Nucleic acid detection refers to a type of technical method for detecting the base characteristics of genetic material DNA or RNA in biological samples (such as microorganisms, viruses, humans, animals, plants, etc.), and the more common ones include qPCR, LAMP, and RPA. Nucleic acid detection usually uses nucleic acid amplification to amplify and enrich the specific sequence of the sample to be tested, and then uses a special method to label it to achieve high-sensitivity and high-specificity detection results. Compared with protein immune technology, nucleic acid detection can accurately distinguish the difference of a single base, which can be used for typing of virus subtypes, and can more accurately identify pathogens and mutations c...

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Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a nucleic acid detection method and a nucleic acid detection system based on the color development of test paper, aiming at solving the problem of poor sensitivity and accuracy of the existing nucleic acid detection method based on color development of test paper The problem

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