An independent sample injection microfluidic immunoassay joint detection device

Abstract

The present invention provides a microfluidic immunoassay joint inspection device for independent sampling, which includes a joint inspection strip, a washing supply unit and a waste liquid absorption and storage unit. The head end of the joint inspection strip is connected to the washing supply unit, and the tail end is connected to the waste liquid Liquid suction storage unit; the first micro-fluid channel and the second micro-fluid channel are recessed on the joint inspection strip, and the joint inspection strip is between the first micro-fluid channel and the second micro-fluid channel A plurality of detection units are set, and each detection unit is provided with a sample storage tank, a marker storage tank and a project reaction detection tank on the joint inspection strip, and the multiple marker storage tanks are respectively equipped with Markers corresponding to multiple targets, solid phase components for capturing multiple targets or corresponding markers are respectively fixed on the inner walls of multiple item reaction detection grooves. The device has high detection sensitivity and avoids mutual interference of multiple items.

Description

technical field [0001] The invention relates to an independent sample injection microfluidic immune analysis joint inspection device, which belongs to the technical field of immune analysis. Background technique [0002] In the process of medical diagnosis, the diagnosis of most diseases requires the joint detection of multiple indicators, and mutual confirmation is needed to assist the diagnosis of the disease, so as to avoid missed diagnosis or misdiagnosis, and to increase the accuracy of the diagnosis. At the same time, multi-index combined detection is also used for clinical differential diagnosis of diseases. For example, in the screening and diagnosis of new coronary pneumonia (COVID-19) caused by the new coronavirus (nCOVI) epidemic, not only should inform the diagnosis of patients or suspicious people Whether the subject is infected with the new coronavirus (nCOVI) or not, if not, the state of the disease caused by other pathogens should be informed as much as possi...

AI Technical Summary

Problems solved by technology

The first is to separate the different detection items on the same nitrocellulose solid-phase membrane (NC membrane), and the markers of different items are layered and stacked together, and the samples penetrate into the marker storage from top to bottom during detection. It binds to the corresponding labeled antigen or antibody on the position, and enters the NC membrane through the contact area with the NC membrane, migrates to the solid phase area by capillary action, and then combines with the solid phase components to form a visible characteristic detection band. However, different items may interfere with each other, especially the corresponding antigen and antibody of a molecule cannot be detected at the same time, and the number of detection indicators is limited at the same time

The second method is to prepare different items into independent joint inspection strips, and then place them in different card slots in the same plastic card, share the sample pool or add samples separately, but this detection efficiency is low

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