Human chondroblastoma cell line and application thereof

Abstract

The invention relates to a human chondroblastoma cell line and application thereof. The preservation number of the human chondroblastoma cell line is CCTCC NO: C2020110, the donor of the human chondroblastoma cell line is from a clinical histone H3.3K36M mutation chondroblastoma patient, lysine at the 36 site on H3F3B is mutated into methionine gene mutation, osteogenesis, chondrogenesis and adipogenesis are hindered in differentiation, and the human chondroblastoma cell line has tibia in-situ tumorigenesis capacity and stable tumorigenesis capacity, can be transfected by lentivirus and has a gene modification function. The human chondroblastoma cell line can be applied to screening of drugs for treating chondroblastoma, construction of drug-resistant chondroblastoma models, construction of chondroblastoma animal models, screening of chondroblastoma related biomarkers and research of the occurrence and development mechanism of chondroblastoma. The invention provides experimental materials for researchers to deeply develop the occurrence and development mechanism of chondroblastoma and find or evaluate treatment schemes.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a human chondroblastoma cell line and its application. Background technique [0002] Chondroblastoma (CB) is a rare benign tumor derived from chondroblasts, accounting for less than 1% of all bone tumors. It occurs more frequently in men, and most patients are between 10 and 25 years old. The ratio of male to female is about (2.0-3.0):1. More than 75% of chondroblastomas involve long bones, with the most common anatomical locations being the epiphyseal and epiphyseal regions of the distal and proximal femur, proximal tibia, and proximal humerus, with similar sites within flat bones such as the acetabulum and ilium Not uncommon; other unusual but classic sites involved are the talus, calcaneus, and patella. In the craniofacial region, the most commonly affected site is the temporal bone. Chondroblastoma almost exclusively involves a single bone and usually occurs in patients with s...

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Problems solved by technology

However, there is no report on the chondroblastoma cell line with histone H3.3K36 gene mutation

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