A human chondroblastoma cell line and its application

Abstract

The present invention relates to a human chondroblastoma cell line and its application. The human chondroblastoma cell line has a deposit number of CCTCC NO: C2020110, and its donor source is a clinical histone H3.3K36M mutant chondroblastoma patient, and the 36th lysine on H3F3B is mutated to methionine Mutation of acid gene, the three-lineage differentiation of osteogenic, chondrogenic and adipogenic is blocked. It has the ability to form tumors in situ of the tibia and has stable tumorigenic ability. It can be transfected by lentivirus and has the function of gene modification. The human chondroblastoma cell line can be used for screening of drugs for the treatment of chondroblastoma, construction of a drug-resistant chondroblastoma model, construction of an animal model of chondroblastoma, screening of chondroblastoma-related biomarkers and research Mechanisms of the development of chondroblastoma. The invention provides experimental materials for researchers to deeply develop the occurrence and development mechanism of human chondroblastoma and to find or evaluate treatment plans.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a human chondroblastoma cell line and its application. Background technique [0002] Chondroblastoma (CB) is a rare benign tumor derived from chondroblasts, accounting for less than 1% of all bone tumors. It occurs more frequently in men, and most patients are between 10 and 25 years old. The ratio of male to female is about (2.0-3.0):1. More than 75% of chondroblastomas involve long bones, with the most common anatomical locations being the epiphyseal and epiphyseal regions of the distal and proximal femur, proximal tibia, and proximal humerus, with similar sites within flat bones such as the acetabulum and ilium Not uncommon; other unusual but classic sites involved are the talus, calcaneus, and patella. In the craniofacial region, the most commonly affected site is the temporal bone. Chondroblastoma almost exclusively involves a single bone and usually occurs in patients with s...

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Problems solved by technology

However, there is no report on the chondroblastoma cell line with histone H3.3K36 gene mutation

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