Nucleic acid detection chip and detection method

A technology for detecting chips and nucleic acids, which is applied in chemical instruments and methods, biochemical equipment and methods, and microbial measurement/inspection. pollution effect

Inactive Publication Date: 2020-09-25
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These technologies can amplify the target signal with high selectivity, but due to the complexity of the operation steps and the limitat

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  • Nucleic acid detection chip and detection method
  • Nucleic acid detection chip and detection method
  • Nucleic acid detection chip and detection method

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Embodiment Construction

[0051] The specific implementation of the nucleic acid detection chip and detection method of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0052] See attached figure 1 , The principle of the nucleic acid detection chip of the present invention is that after the detection sample is passed through the lysis pool 2, the cleaning pool 3, and the elution pool 4 to obtain nucleic acid substances, after the amplification reaction and the CRISPR reaction, the nucleic acid analysis and confirmation are completed through detection. In the figure, the lysis pool 2 , the cleaning pool 3 , and the elution pool 4 are classified as the channel microfluidic part 19 , while the detection part is controlled by the droplet drive module 12 .

[0053] See attached figure 2The nucleic acid detection chip includes a closed shell 1, and the detection process is carried out in the shell. A cracking pool 2, a cleaning pool 3, an elution poo...

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Abstract

The invention relates to the technical field of biology, and discloses a nucleic acid detection chip. The chip comprises a cracking tank, a cleaning tank, an elution tank and a reaction detection unitwhich are sequentially arranged in a closed shell, a sample input port is formed in the cracking tank, an isolation channel is arranged between the cracking tank and the cleaning tank, an isolation channel is also arranged between the cleaning tank and the elution tank, the elution tank communicates with the reaction detection unit, magnetic beads are arranged in the cracking tank, a blocking liquid is arranged in the isolation channels, a magnet unit is arranged outside the shell, and the magnet unit drives the magnetic beads in the chip to move among the cracking tank, the cleaning tank andthe elution tank through the isolation channels. The invention also discloses a method for performing nucleic acid detection by using the nucleic acid detection chip. According to the invention, microfluidic and CRISPR detection technologies are combined, and multi-target, high-sensitivity and automatic detection of pathogenic microorganism nucleic acid signals is realized on a single chip.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nucleic acid detection chip and a detection method. Background technique [0002] Establishing an accurate, rapid and portable nucleic acid detection method for on-site rapid detection of microorganisms, viruses and other biological pathogens is of great significance to the prevention and treatment of highly infectious diseases. [0003] At present, many clinical nucleic acid detection technologies still lack detection equipment and kits for high-risk and highly infectious pathogens that do not rely on high-level biosafety laboratories. The general nucleic acid detection process needs to be amplified to obtain a sufficient amount of nucleic acid to be detected, and then use fluorescence, chromogenic or potential signals as a reporter system. Current nucleic acid amplification techniques include variable temperature amplification techniques such as polymerase chain reaction (polyme...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/34C12M1/00C12Q1/6844B01L3/00
CPCB01L3/50273B01L3/502753C12Q1/6844B01L2300/0861B01L2200/0647B01L2200/10B01L2300/0887B01L2300/165B01L2400/0415B01L2400/0433C12Q2523/308C12Q2563/159C12Q2521/327C12Q2525/161C12Q2565/607C12Q2527/101
Inventor 张立新王慧锋顾震刘光颜秉勇黄术强田晓丽黄高健刘家坤韩照晰
Owner EAST CHINA UNIV OF SCI & TECH
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