Nucleic acid detection chip and detection method

Abstract

The invention relates to the technical field of biology, and discloses a nucleic acid detection chip. The chip comprises a cracking tank, a cleaning tank, an elution tank and a reaction detection unitwhich are sequentially arranged in a closed shell, a sample input port is formed in the cracking tank, an isolation channel is arranged between the cracking tank and the cleaning tank, an isolation channel is also arranged between the cleaning tank and the elution tank, the elution tank communicates with the reaction detection unit, magnetic beads are arranged in the cracking tank, a blocking liquid is arranged in the isolation channels, a magnet unit is arranged outside the shell, and the magnet unit drives the magnetic beads in the chip to move among the cracking tank, the cleaning tank andthe elution tank through the isolation channels. The invention also discloses a method for performing nucleic acid detection by using the nucleic acid detection chip. According to the invention, microfluidic and CRISPR detection technologies are combined, and multi-target, high-sensitivity and automatic detection of pathogenic microorganism nucleic acid signals is realized on a single chip.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nucleic acid detection chip and a detection method. Background technique [0002] Establishing an accurate, rapid and portable nucleic acid detection method for on-site rapid detection of microorganisms, viruses and other biological pathogens is of great significance to the prevention and treatment of highly infectious diseases. [0003] At present, many clinical nucleic acid detection technologies still lack detection equipment and kits for high-risk and highly infectious pathogens that do not rely on high-level biosafety laboratories. The general nucleic acid detection process needs to be amplified to obtain a sufficient amount of nucleic acid to be detected, and then use fluorescence, chromogenic or potential signals as a reporter system. Current nucleic acid amplification techniques include variable temperature amplification techniques such as polymerase chain reaction (polyme...

AI Technical Summary

Problems solved by technology

These technologies can amplify the target signal with high selectivity, but due to the complexity of the operation steps and the limitations of equipment size, they are mainly used in professional laboratory applications, and it is difficult to carry out field application

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