A plant disease resistance inducer and its application

A kind of inducer and plant technology, applied in the direction of plant growth regulator, plant growth regulator, application, etc., can solve the problem of limited response ability of the immune and disease resistance system, and achieve the effect of improving disease resistance

Active Publication Date: 2020-03-24
SHANDONG AGRICULTURAL UNIVERSITY
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the response ability of the plant's own immune system is limited, so plant resistance inducers are needed to enhance their disease resistance
The currently known plant disease resistance inducers can be divided into two categories: non-biological sources and biological sources. Among them, the inducers of non-biological sources include inorganic salts, such as mercuric chloride, copper sulfate, etc., and organically synthesized inducers. The oligosaccharide inducer obtained by the method; the inducer of biological origin includes the oligosaccharide inducer, protein inducer and glycoprotein isolated from the metabolites of fungi or extracted from mycelium inducers, but there are no reports of nucleotide substances inducing plant disease resistance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A plant disease resistance inducer and its application
  • A plant disease resistance inducer and its application
  • A plant disease resistance inducer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Experimental crop: Arabidopsis (for Col-0 background).

[0036] 2. Planting method: Arabidopsis thaliana is planted in a greenhouse with 16 hours of sunshine and 8 hours of darkness. The cultivation temperature is 21°C and the air humidity is 60-70%. laboratory.

[0037] 3. Test method:

[0038] When Arabidopsis was cultivated to the 4th week, the plant disease resistance inducer (2'-deoxyguanosine concentration was 100ng / ml, and the volume percentage of Tween-20 was 0.01%) was sprayed on Arabidopsis leaves At the same time, only 0.01% (volume percentage) Tween-20 aqueous solution was sprayed as a control. After 2 hours, the leaves were taken for active oxygen detection, and RNA was extracted to detect the expression level of PR1 gene; at the same time, Pseudomonas syringae pv tomato DC3000 (Pseudomonas syringae pv tomatoDC3000, DC3000) was inoculated. Three days later, observe and take pictures, and get the diseased leaves to make a bacterial growth curve, and ca...

Embodiment 2

[0048] 1. Experimental crop: rice (japonica rice Zhonghua 11).

[0049] 2. Planting method: Rice is planted in a greenhouse with 16 hours of sunlight and 8 hours of dark cultivation, the cultivation temperature is 28°C at room temperature, and the air humidity is 60-70%; the greenhouse is located in the State Key Laboratory of Crop Biology, Shandong Agricultural University .

[0050] 3. Test method:

[0051] When the rice is cultivated to the 8th week, the plant disease resistance inducer (the concentration of 2'-deoxyguanosine is 100ng / ml, and the volume percentage of Tween-20 is 0.01%) is sprayed on the rice leaves. Only 0.01% Tween-20 aqueous solution was sprayed as a control. 2 hours later, inoculate Xanthomonas oryzae pv.Oryzicola, Xoc physiological race RS105, during the onset period, spray the plant resistance inducer again every four days, and the control group spray 0.01% Tween again every four days -20 aqueous solution. After 14 days, the lesion was photographed,...

Embodiment 3

[0056] 1. Experimental crop: rice (japonica rice Zhonghua 11).

[0057] 2. Planting method: Rice is planted in a greenhouse with 16 hours of sunlight and 8 hours of dark cultivation, the cultivation temperature is 28°C at room temperature, and the air humidity is 60-70%; the greenhouse is located in the State Key Laboratory of Crop Biology, Shandong Agricultural University .

[0058] 3. Test method:

[0059] When the rice is cultivated to the 10th week, the plant disease resistance inducer (2'-deoxyguanosine concentration is 100ng / ml, the volume percentage of Tween-20 is 0.01%) is sprayed on the stem part of the rice, At the same time, only 0.01% Tween-20 aqueous solution was sprayed as a control. After 2 hours, inoculate sheath blight bacteria on the leaf sheath and keep it moisturized, observe the disease situation in 3-4 days, cut off the whole rice plant after the disease, and take pictures of the disease spot phenotype.

[0060] 4. Test results:

[0061] see results ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a plant disease-resistant resistance inducer and application thereof. Discovered that 2'-deoxyguanosine can directly cause active oxygen burst of plants and up-regulated expression of a disease-resistant gene PR1, improves the disease resistance of the plants to pathogenic bacteria and exerts the effect of the plant disease-resistant resistance inducer. 2'-deoxyguanosine iswide in source, has the characteristics of high efficiency, non pollution, low cost and the like, has a large application potential as a novel plant disease-resistant resistance inducer and accords with the requirement for sustainable and healthy development of agriculture.

Description

technical field [0001] The invention relates to the technical field of biological pesticides, in particular to a plant disease resistance inducer and its application. Background technique [0002] Crop disease is one of the natural disasters that endanger agricultural production, national economic development and people's living standards. It causes a loss of 10-15%. It is a key factor affecting stable, high-yield, and high-quality crops. Pathogenic bacteria can produce toxic substances after infection, which limits crop cultivation and storage and transportation of agricultural products, and directly reduces the yield and quality of agricultural products. Farmers have to spray pesticides to prevent and control the occurrence and development of diseases, so as to avoid or reduce the interference of pathogenic bacteria on the physiological functions of crops. Pesticides can indirectly cause damage to the ecological balance through the food chain, the enrichment of pesticides...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P21/00A01P1/00A01P3/00
CPCA01N43/90
Inventor 丁新华储昭辉路冲冲
Owner SHANDONG AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap