The present invention relates to the technical field of fruit tree planting, and particularly discloses a cultivation method of seedless Citrus reiculata Blanco. The method comprises the following steps of S1, field selection; S2, seedling selection; S3, land preparation; S4, field planting; S5, water and fertilizer management; S6, disease and pest management; S7, shaping management; and S8, flower and fruit management. According to the method, 091 seedless Citrus reiculata Blanco cup seedlings are selected, measures of water and fertilizer management, shaping management, fruit management andthe like are optimized, and summer tip removing, autumn tip nourishing, flower promoting, flower nourishing, flower retention, fruit retention, nourishing, sterilizing, pest killing and the like are organically combined, so that sufficient nutrition can be provided for growth of the seedless Citrus reiculata Blanco, excessive growth of branches and leaves can be avoided, the fruit yield and quality are improved, the commodity rate and the economic benefit of the seedless Citrus reiculata Blanco are increased, and planting popularization of the seedless Citrus reiculata Blanco is facilitated.