Use of anti-aging and anti-oxidation compounds in the preparation of medicines or anti-aging cosmetics
A technology of compounds and cosmetics, which is applied in the field of anti-aging and anti-oxidation compounds, preparation of drugs or anti-aging cosmetics, can solve the problems of limited opportunities for selection, prolongation, and insufficient variety and types of drugs, etc., and achieve good commercial value, Prevent cell aging, good anti-oxidation effect
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Embodiment 1
[0027] The preparation of embodiment 1 formula (I) compound
[0028] 0.73ml of 30% H at 0°C 2 o 2 The solution was added to a solution of 1 g of 4-bromoanisole in 5 ml of acetic acid. The mixture was stirred at RT for 12 h and worked up with 10% NaOH solution. The aqueous phase was extracted with ethyl acetate. The organic phase was washed with saturated NaCl solution, washed with Na 2 SO 4dry. Removal of solvent afforded 0.99 g of crude A. 223 mg of sodium azide was added dropwise to a solution of 900 mg of product A in 18 ml of chloroform at 0°C. The mixture was stirred at 0 °C for 12 h and ice water was added. Conventional workup afforded 592.6 mg of product B. 253 mg 3-hydroxy-2-oxo-pyrrolidine-3-carboxylic acid-3-chloro-5-fluoro-benzylamide, 359 mg potassium carbonate, 382 mg N',N'-dimethyl-ethane-1,2 -Diamine and 330 mg of copper iodide were added successively from a solution of 200 mg of product B prepared in the above step in 12 ml of degassed dioxane. The m...
Embodiment 2
[0030] Example 2 Effects of compounds on the expression of ROS in human epidermal keratinocytes
[0031] (1) Take human epidermal keratinocytes in the logarithmic growth phase (Product No. 2110, Sciencell), and adjust the density to 5×10 5 / mL, add to 6-well plate (100μL / well), 37℃, 5% CO 2 Adhesive culture.
[0032] (2) The compound prepared in Example 1 was diluted into a series of concentrations: 1, 10, 50 μg / mL, and quercetin was used as a positive control with a concentration of 50 μg / mL. Serum-free medium was used as a negative control group.
[0033] (3) After 24 hours of wall attachment, the compound was added. After incubation for 12 h, the original medium was discarded, and serum-free medium containing DCFH-DA (20 mM) was added to each well, except for the blank group.
[0034] (4) Use a multi-functional microplate reader to immediately measure the fluorescence value, excitation wavelength (485 nm), emission wavelength (538 nm), at 37°C, detect every 5 min, and ...
Embodiment 3
[0037] Example 3 The inhibitory effect of the compound on the MAPK pathway
[0038] Human epidermal keratinocytes were cultured, and the cells were collected at a density of 5×10 5 individual / mL. First, human epidermal keratinocytes were inoculated into Transwell cell chambers (2 mL / well). Add PBS (2 mL / well), select the irradiation dose, the irradiation dose is 100 μj / cm 2 Irradiate for 2 min. After the irradiation, the expression level of p38MAPK was partially detected. Concentration of the compound was added partly, and the small chamber in the Transwell plate was placed in a 6-well plate with HFF cells. After incubation for 24 h, the cells were taken to measure the expression level of p38MAPK. Normal non-irradiated epidermal keratinocytes were used as a negative control, and the results were as follows figure 2 shown. from figure 2 It can be seen that 50 μg / mL compound can significantly reduce the MAPK-related factor p38MAPK in human epidermal keratinocytes. Co...
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