Probe composition, reagent and control system for TERT gene all-exon next-generation sequencing

A next-generation sequencing, whole-exome technology, applied in specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, biomass post-processing, etc. The problem of high cost, to achieve the effect of simple operation and low cost

Pending Publication Date: 2020-10-13
CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the TERT gene mutation detection in the prior art is to detect the C228T and C250T sites of the TERT gene promoter activity, which has the following limitations: 1. Real-time fluorescent quantitative PCR detection and gene ch...

Method used

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  • Probe composition, reagent and control system for TERT gene all-exon next-generation sequencing
  • Probe composition, reagent and control system for TERT gene all-exon next-generation sequencing
  • Probe composition, reagent and control system for TERT gene all-exon next-generation sequencing

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Embodiment 1

[0024] The probe composition used for the second-generation sequencing of the whole exon of the TERT gene includes the sequences shown in SEQ ID NO: 01 to SEQ ID NO: 36.

[0025] Dilute the 36 probes according to the standard of 100p per tube, take a new 1.5ml centrifuge tube, add 2ul of each probe respectively, and replenish water to a total volume of 200ul. Maintain the final concentration at 1 pmol, shake and mix well, and store at -20°C for later use.

[0026] The reagents used for next-generation sequencing of whole exons of TERT gene include the above-mentioned probe composition and reagents required for next-generation sequencing, and the specific content is described in the following method for second-generation sequencing of whole exons of TERT gene.

[0027] The method for second-generation sequencing of the whole exons of the TERT gene is to use the above-mentioned reagents to perform second-generation sequencing of the whole exons of the TERT gene, including the fo...

Embodiment 2

[0122] The second-generation sequencing method for the whole exon of the TERT gene described in Example 1 is implemented by controlling the special equipment through the second-generation sequencing control system for the whole exon of the TERT gene.

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Abstract

The invention provides a probe composition, reagent and control system for TERT gene all-exon next-generation sequencing. The probe composition for TERT gene all-exon next-generation sequencing comprises sequences as shown in SEQ ID NO:01 to SEQ ID NO:36. The probe composition, the reagent and the control system for TERT gene all-exon next-generation sequencing provided by the invention solve thefollowing existing technical problems: 1, point mutation of a TERT gene is mainly detected in the prior art, so that the detection range is limited; 2, real-time fluorescent quantitative PCR detectionis performed, so that a gene chip can only detect known sites; 3, the Sanger sequencing test length is smaller than 800 bp; 4, low-frequency mutation cannot be detected; and 5, the operation is tedious and the cost is high when a plurality of sites are detected.

Description

technical field [0001] The invention relates to the field of gene sequencing, in particular to a probe composition, reagent and control system for second-generation sequencing of whole exons of TERT gene. Background technique [0002] TERT (telomerase reverse transcriptase, telomerase reverse transcriptase) refers to the catalytic subunit with reverse transcriptase activity in telomerase, which is an important catalytic part of telomerase and affects the regulation mechanism of telomeres, thereby maintaining cell Immortalization and carcinogenesis. The TERT gene is located on chromosome 5, and its transcript NM_001193376.1 has 15 exons, with a total length of 3275 bp. TERT gene mutations are found in malignant tumors such as melanoma, lipoma, hepatoma, and glioma All have a certain detection rate, and the prognosis of positive patients is mostly poor, and they are biomarkers of related tumors. [0003] Regarding the mutation of the TERT gene, it is known that there are two...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869C12N15/11C12M1/36C12M1/34
CPCC12Q1/6869C12Q2531/113C12Q2535/122C12Q2537/165
Inventor 代冰余艳周鹏涛周梅华
Owner CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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