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Detection method and application forfingerprint spectrum of Qijudihuang pills

A technology of Qiju Dihuang Pills and fingerprints, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of lack of qualitative and quantitative research on Qiju Dihuang Pills

Active Publication Date: 2020-10-27
HEHUANG PHARMA SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is a lack of systematic qualitative and quantitative research on Qiju Dihuang Pills

Method used

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  • Detection method and application forfingerprint spectrum of Qijudihuang pills
  • Detection method and application forfingerprint spectrum of Qijudihuang pills
  • Detection method and application forfingerprint spectrum of Qijudihuang pills

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preparation example Construction

[0112] Preparation of the test solution: after pulverizing Qiju Dihuang Wan, accurately weigh 0.8g of Qiju Dihuang Wan powder, place it in a 50mL conical flask with a stopper, and accurately add 24-24 26ml, weighed, sealed with an ultrasonic instrument at a power of 200-300W and a frequency of 30-50kHz for 20-40min, let it cool, weighed again, and used 50-100% methanol aqueous solution to make up for the loss weight, take the supernatant, and filter it with a microporous membrane with a pore size of 0.22 μm to obtain the test solution.

[0113] Preparation of reference solution: Accurately weigh 5-hydroxymethylfurfural, morroniside, chlorogenic acid, loganin, paeoniflorin, luteolin, 3,5-O-dicaffeoylquinic acid, dandelion Cortex phenol, 23-acetyl alisitol B reference substances were placed in volumetric flasks for gradual dilution. That is, first add 50-100% methanol aqueous solution to dissolve and shake to a constant volume in a volumetric flask to obtain a stock solution of...

Embodiment 1

[0146] 1. Sample pretreatment

[0147] Preparation of the test solution: After pulverizing Qiju Dihuang Pills, accurately weigh 0.8g of Qiju Dihuang Pills powder, place in a 50mL conical flask with a stopper, accurately add 25ml of methanol aqueous solution with a volume percentage concentration of 80%, weigh Weight, after the seal is sealed, use an ultrasonic instrument to extract ultrasonically for 30 minutes at a power of 250W and a frequency of 40kHz, let it cool, and weigh again, use 80% methanol aqueous solution to make up the lost weight, take the supernatant, and use a pore size of 0.22 μm microporous membrane to obtain the test solution.

[0148] Preparation of reference solution: Accurately weigh 5-hydroxymethylfurfural, morroniside, chlorogenic acid, loganin, paeoniflorin, luteolin, 3,5-O-dicaffeoylquinic acid, dandelion Cortex phenol, 23-acetyl alisitol B reference substances were placed in volumetric flasks for gradual dilution. Promptly add 80% methanol aqueous...

Embodiment 2

[0172] Adopt the detection method of the Qiju Dihuang Pills fingerprint spectrum set up in above-mentioned embodiment 1 to detect 10 batches of Qiju Dihuang Pills, obtain the fingerprint spectrum of need testing solution and the fingerprint spectrum of reference substance solution, the batch number of 10 batches of Qiju Dihuang Pills See Table 4. The obtained fingerprint data of the test product was imported into the software of "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" 2009 version issued by the National Pharmacopoeia Committee, and compared with the standard fingerprint of Qiju Dihuang Pills obtained under the same fingerprint detection conditions The similarity between the fingerprint of Qiju Dihuang Pills and the standard fingerprint of Qiju Dihuang Pills ≥ 0.95 is taken as the standard, and the S1 sample in Table 4 is used as the reference spectrum. figure 1 , see the overlay map figure 2 . The standard fingerprints of Qiju Dihuang Pi...

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Abstract

The invention provides a detection method for a fingerprint spectrum of Qijudihuang pills. The invention also provides application of the detection method forthe fingerprint spectrum of the Qijudihuang pills in quality detection of components in the Qijudihuang pills and a quality detection method thereof. The invention further provides a method for determining the contents of the nine componentsin the Qijudihuang pills. The invention further provides a screening method of the fingerprint spectrums of the multiple medicinal materials in the Qijudihuang pills. The invention provides a detection method and application forthefingerprint spectrum of Qijudihuang pills. The high performance liquid chromatography of the Qijudihuang pill is established; 9 chemical components in the Qijudihuang pill can be quantitatively analyzed; chromatographic peaks of each single medicinal material in the Qijudihuang pill are affiliated and confirmed, effective monitoring can be performed from the source of the raw materials, the quality of the raw medicinal materials is strictly controlled, and the quantity of effective components in the Qijudihuang pill is relatively stable, so that the safety and effectiveness of clinical medication are ensured.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicine components, and relates to a method for detecting the fingerprint of Qiju Dihuang Pills and its application, in particular to a method for detecting the fingerprint of Qiju Dihuang Pills and its application in quality detection. Background technique [0002] Qiju Dihuang Pill is a drug for nourishing the kidney and nourishing the liver. It is clinically used for liver and kidney yin deficiency, dizziness and tinnitus, photophobia, tears in the wind, and blurred vision. Although Qiju Dihuang Pills reveals the composition of its medicinal materials, which are composed of 8 herbs, namely wolfberry, rehmannia glutinosa, moutan cortex, poria cocos, chrysanthemum, wine cornel, yam and Alisma, but its chemical composition is extremely complex. In the 2020 edition of the "Pharmacopoeia of the People's Republic of China", only the properties, microscopic identification and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8686G01N2030/047
Inventor 沈丹萍张正光詹常森姜鹏温方方
Owner HEHUANG PHARMA SHANGHAI
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