Large-scale preparation method of F genotype mumps attenuated live vaccine

A live attenuated vaccine, mumps technology, applied in biochemical equipment and methods, inactivation/attenuation, medical preparations containing active ingredients, etc.

Pending Publication Date: 2020-10-30
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the prior art, there is no relevant report on the large-scale preparation of F genotype mumps live attenuated vaccine by using cell factories and human diploid cells at the same time

Method used

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  • Large-scale preparation method of F genotype mumps attenuated live vaccine
  • Large-scale preparation method of F genotype mumps attenuated live vaccine
  • Large-scale preparation method of F genotype mumps attenuated live vaccine

Examples

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Embodiment 1

[0035] Example 1: Large-scale preparation of F genotype mumps live attenuated vaccine

[0036] All operations are performed in a sterile clean bench. The matrix fluid used to prepare the solution is water for injection. Every 1000mL MEM medium contains 9.4g MEM dry powder.

[0037] 1. Recovery, passage and counting of human diploid cells

[0038] 1.1 Preparation of MEM culture medium: Take a UPV tank containing 20400mL of MEM medium pre-warmed to 37°C, connect the short tube for ventilation of the UPV tank to the suction tube of the vacuum pressure pump, and connect the long tube for the outlet of the UPV tank to the non-scale glass Connect the suction pipe, and inhale 720mL of 6.6% sodium bicarbonate, 480mL of 3% glutamine, and 2400mL of newborn bovine serum in sequence with negative pressure. After the solution is extracted, use another long tube connected to an air filter on the UPV tank to ventilate under positive pressure for at least 30 seconds and mix well to obtain ...

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Abstract

The invention discloses a large-scale preparation method of an F genotype mumps attenuated live vaccine. The preparation method comprises the following steps: passage and counting of KMB-17 cells, preparation of a virus harvesting liquid, preparation of a vaccine stock solution, preparation of a vaccine semi-finished product and preparation of a finished product. The F genotype mumps virus attenuated live vaccine is prepared on a large scale by taking human diploid cells as a matrix in a cell factory for the first time, so that the production cost can be reduced and the production time can besaved while the safety and the quality stability of the vaccine are ensured. Compared with traditional Roche flask culture, the method has the advantages that the amount of required virus seeds is small, 60000 vaccine finished products can be obtained only by 60-84 mL of virus seeds in each batch, the yield is high, the cost is low, the quality is stable, and the like, and the method has good application prospects in preventing and controlling infection and transmission of epidemic parotitis in China.

Description

technical field [0001] The invention belongs to the technical field of antiviral vaccine preparation, and in particular relates to a cell factory as a culture container, and uses human diploid cells (KMB-17 strain) to carry out adaptive subculture and growth, and then realizes large-scale preparation of F genotype Methods of Live Attenuated Mumps Vaccine. Background technique [0002] Mumps virus (MuV) is the pathogen that causes mumps. Mumps virus belongs to the family Paramyxoviridae. The genome is a single negative-strand ribonucleic acid (RNA) without segments, containing about 15,500 nucleotides. The sequence of -L-5' aligns the genes encoding seven viral proteins. Among them, the degree of variation of the SH gene is the largest, so the SH gene is generally used as the basis for typing. According to the differences of nucleotides on the SH antigen, mumps virus can be divided into 12 genotypes, A-L. my country is a region where a large number of live attenuated mump...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/165A61P31/14C12N5/071C12N7/08
CPCA61K39/12A61P31/14C12N5/0688C12N7/00A61K2039/5254C12N2760/18751C12N2760/18764
Inventor 李琦涵王晶晶刘龙丁车艳春李卫东梁燕王微张莹杨伟邱恒杨振清
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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