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Recombinant virus capable of stably expressing target proteins

A technology of recombining viruses and viruses, applied in the field of molecular biology, can solve problems that affect the efficacy of VLP yields and cannot be maintained stably, etc.

Pending Publication Date: 2020-11-06
BOEHRINGER INGELHEIM VETMEDICA CHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, it was found that the P1-2A gene is not stably maintained during serial passaging of recombinant viruses such as adenovirus, which thus negatively affects VLP yield and vaccine efficacy

Method used

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  • Recombinant virus capable of stably expressing target proteins
  • Recombinant virus capable of stably expressing target proteins
  • Recombinant virus capable of stably expressing target proteins

Examples

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Embodiment Construction

[0015] The present invention relates to a recombinant virus comprising an expression cassette in the genome of the recombinant virus, wherein the expression cassette comprises a first polynucleotide encoding at least one polypeptide of interest, and it is an essential gene of the recombinant virus or a functional fragment thereof and wherein the first polynucleotide is functionally linked to the second polynucleotide, wherein the first polynucleotide is located upstream of the second polynucleotide, and wherein the The second polynucleotide is the only copy of said essential gene or functional fragment thereof that is active in the recombinant virus.

[0016] The present invention also relates to a method for preparing a recombinant virus, which includes constructing an expression cassette in the genome of the recombinant virus, wherein the expression cassette comprises a first polynucleotide encoding at least one polypeptide of interest, and it is a polynucleotide of the recom...

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Abstract

Provided is a method to generate a recombinant virus that can stably express target proteins. The recombinant virus of the present invention is useful for producing an immunogenic composition or vaccine.

Description

technical field [0001] The invention relates to the field of molecular biology, and in particular to a recombinant virus capable of stably expressing a target protein and its use. Background technique [0002] Foot-and-mouth disease virus (FMDV) is a ribonucleic acid (RNA) virus belonging to the genus Aphthovirus of the family Picornaviridae (Cooper et al., Intervirology, 1978, 10, 165-180). As a naked icosahedral virus with a diameter of about 25 nm, FMDV contains a single-stranded RNA molecule consisting of about 8500 nucleotides with positive polarity. This RNA molecule comprises a single open reading frame (ORF) that encodes a single polyprotein including, inter alia, the capsid precursor (also known as protein P1). Protein P1 is myristoylated at its amino terminus. During the maturation process, protein P1 is cleaved by protease 3C into three proteins called VPO, VP1 and VP3 (Belsham G.J., Progress in Biophysics and Molecular Biology, 1993, 60, 241-261). In the virio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/09A61K39/00A61K48/00A61P31/00
CPCA61P31/00A61K39/12A61K2039/5256A61K2039/552C12N2770/32134C12N7/00C07K14/005C12N2770/32121C12N2770/32122C12N2770/32123C12N2710/16722A61P31/12C12N2710/16734
Inventor 马光刚袁森
Owner BOEHRINGER INGELHEIM VETMEDICA CHINA CO LTD
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