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A method for improving the effect of intravenous injection in newborn mice

A newborn mouse and vein technology, which is applied in the fields of equipment for restraining animals, medical science, instruments, etc., can solve problems such as not meeting the requirements of drug research and development, quality and rigor defects, and achieve the goal of improving the success rate and ensuring the blood flow rate Effect

Active Publication Date: 2021-07-27
蓝图生物医药(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to eliminate this kind of misjudgment, some researchers have increased the accuracy of judgment by adding an indicator that has a clear contrast with the blood color to the injection, but this operation introduces the impact of the color value reagent on the evaluation of the mouse model, which is not in line with the drug R & D requirements
Therefore, there are serious defects in the quality and rigor of the neonatal mouse model for early intervention evaluation of drugs obtained through the existing intravenous injection method, which needs to be optimized urgently

Method used

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  • A method for improving the effect of intravenous injection in newborn mice
  • A method for improving the effect of intravenous injection in newborn mice
  • A method for improving the effect of intravenous injection in newborn mice

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Experimental program
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Effect test

Embodiment 1

[0042] A high-precision intravenous injection method for neonatal mice, including:

[0043] Preprocessing:

[0044] S1) Disinfect the operating table and related equipment, and pre-cool the operating table;

[0045] S2) Prepare the injection solution and the syringe.

[0046] Neonatal mouse injections:

[0047] S3) anesthetize the newborn mouse, and fix the mouse on the operating table;

[0048]S4) Place the fixed mouse under the dissecting microscope, align the focus, and use the LED light to illuminate from the front of the operator. The brightness of the light is 600-700lx, and a slit at the bottom is placed between the light and the animal. The light partition allows a small part of the light to pass through the slit, forming a diffraction effect ( figure 1 );

[0049] S5) dripping 300ul sterile water on the face above the facial vein of the mouse to form a liquid focusing lens;

[0050] S6) Using the syringe prepared in advance, select any vein in the field of view ...

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Abstract

The invention discloses a method for improving the effect of intravenous injection of newborn mice. A light-shielding board is arranged between them, and a light-transmitting gap is arranged between the light-shielding board and the platform, and the transmitted light is irradiated on the head of the newborn mouse from an obliquely downward direction. In some examples of the present invention, by controlling the light conditions, the blood flow details in the superficial temporal vein of neonatal mice can be clearly observed, and whether the injection is successful can be directly observed without the aid of dyes. The harm to newborn mice caused by large-dose injection in the existing method is avoided.

Description

technical field [0001] The invention relates to a modeling method for mice, in particular to a method for improving the effect of intravenous injection of newborn mice. Background technique [0002] There are a variety of genetic rare diseases that commonly occur in infancy, seriously threatening the life and health of newborns, such as spinal muscular atrophy, Duchenne muscular dystrophy, etc. At present, there are almost no effective treatments or drugs for these diseases, so related The development of therapeutic drugs is urgently needed. These diseases are mainly early onset. In the non-clinical research and development stage, early drug intervention research is the most suitable. In the application research of various mouse disease models, the administration method should simulate the clinical administration conditions as much as possible. Intravenous injection is the most widely used Therefore, an effective neonatal mouse research model administered by intravenous inj...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/06A61D7/00A61D3/00
CPCA61D3/00A61D7/00A61D2003/003
Inventor 高堂鑫余珊
Owner 蓝图生物医药(广州)有限公司