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Application of ellagic acid metabolite urolithin A in the preparation of anti-enteroviral drugs

An enterovirus and drug technology, applied in the direction of antiviral agents, resistance to vector-borne diseases, pharmaceutical formulations, etc., can solve the problem of no relevant research reports on antiviral effects

Active Publication Date: 2021-08-03
ZUNYI MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Biological activity studies have shown that these intestinal microbial metabolism has various biological activities such as anti-oxidation, anti-inflammation, anti-tumor and inhibition of protein glycosylation, but there is no relevant research report on anti-viral effects

Method used

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  • Application of ellagic acid metabolite urolithin A in the preparation of anti-enteroviral drugs
  • Application of ellagic acid metabolite urolithin A in the preparation of anti-enteroviral drugs
  • Application of ellagic acid metabolite urolithin A in the preparation of anti-enteroviral drugs

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Experimental program
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Embodiment Construction

[0022] The following is further described in detail through specific implementation methods:

[0023] In the following description, Urolithin A is abbreviated as UroA, and its structural formula is as follows figure 1 shown.

[0024] 1 Detection of the toxicity of Ribavirin and UroA to cells

[0025] The toxicity of Ribavirin and UroA to cells was detected by CCK8 method. The cells were seeded in a 96-well cell culture plate. When the cell density reached 70%, the cells were washed once with PBS, and the drug concentrations of 100 μM, 50 μM, 25 μM, 12.5 μM, 6.25 μM, 3.125 μM and 0 μM were added to the cell culture wells. Cell maintenance medium (containing 1% serum and 1% penicillin and streptomycin) 100 μL. Four replicates were performed for each drug concentration. Maintain culture (5% CO 2 and 37°C culture conditions) 48 hours later, 10 μL of CCK8 was added to each well, and the OD value was measured at a wavelength of 450 nm. The OD value of 0 μM drug concentration i...

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Abstract

The application discloses the application of ellagic acid metabolite Urolithin A in the technical field of pharmaceutical preparations in the preparation of anti-enterovirus drugs, especially the application in the preparation of drugs for preventing and treating enterovirus 71 (Enterovirus 71, EV71). The present invention shows that Urolithin A can inhibit the replication of EV71 at gene and protein levels through the influence of UroA on EV71 virus titer and EV71 protein expression. It is proved that the Urolithin A has obvious anti-EV71 effect and can be used to prepare anti-EV71 medicine.

Description

technical field [0001] The technical field of pharmaceutical preparations of the present invention particularly relates to the application of ellagic acid metabolite Urolithin A in the preparation of anti-enterovirus drugs. Background technique [0002] Hand, foot, and mouth disease (HFMD) is a common infectious disease in children worldwide caused by human enterovirus (HEV), with a high incidence in children under 5 years old. HFMD is mostly a self-limiting disease, accompanied by symptoms such as fever, rash on hands and feet, and oral herpes. However, in some patients, the disease progresses rapidly, which can lead to severe pulmonary edema, neurological and systemic complications, and even death. Enterovirus 71 (human enterovirus 71, EV71), a member of HEV, is a single-stranded positive-sense RNA virus belonging to the family Picornaviridae, and is the main pathogen that causes severe and fatal cases of HFMD. Since 1997, HFMD has broken out in the Asia-Pacific region. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/366A61P31/14
CPCA61K31/366A61P31/14Y02A50/30
Inventor 王盛羽孙欣何灿粲乔俊华田浪飞陈雅萍
Owner ZUNYI MEDICAL UNIVERSITY
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