Drug target expression and purification method for high blood pressure
A purification method and high blood pressure technology, which is applied in botany equipment and methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of large side effects and lack of specificity of antihypertensive drugs, and achieve the promotion of cell growth , reduce cell aggregation, and promote the effect of research and development
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[0025] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described below in conjunction with specific embodiments. It should be understood that the specific embodiments and related drawings described here are only used to explain the present invention, not to limit the present invention.
[0026] 1. Expression of drug targets
[0027] ①Construction of drug target expression plasmid
[0028] The human full-length TRPM4 construct, whose amino acid sequence is shown in Sequence No.1, was cloned into the pAntigenMem vector, and the pAntigenMem vector map is shown in figure 1 shown. A maltose-binding protein (MBP) tag (to improve the solubility of the drug target) was added to its N-terminus. The constructed recombinant plasmid was transformed into DH5α competent cells, plated on LB plates containing a certain concentration of antibiotics, colonies were selected, plasmids were extracted, a...
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