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Single cell mass spectrometry device and method

A technology of mass spectrometry analysis and analysis method, which is applied in the field of single-cell mass spectrometry analysis devices, can solve the problems of unstable formation of picoliter single droplets, inability to form ionized spray, and low efficiency of mass spectrometry detection, so as to improve sensitivity and detection efficiency, Achieve full automation and improve analysis efficiency

Active Publication Date: 2021-02-05
CHINA INNOVATION INSTR CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] A. Picogradation single droplet formation is unstable, resulting in the inability to form an ionized spray, and a single operation is invalid;
[0008] B. After absorbing the extract, it needs to be rotated about 225° to turn the capillary to the mass spectrometer inlet. This technical means makes the droplet move to the other end of the capillary needle under the influence of gravity when the needle is facing upward, resulting in the droplet rotating to the mass spectrometer port. Greatly reduced, the detection efficiency of mass spectrometry is low, and even no signal can be detected

Method used

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  • Single cell mass spectrometry device and method
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  • Single cell mass spectrometry device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The single-cell mass spectrometry device of Example 1 of the present invention, the single-cell mass spectrometry device includes:

[0042] A carrier and a mass spectrometer, the carrier is used to carry a plurality of mutually separated cells, and the mass spectrometer has a sample inlet;

[0043] A transmission unit, the transmission unit includes a capillary needle, a moving part, a negative pressure generating module, a driving module and a support; the capillary needle has openings at both ends and is arranged on the support; when the moving part is in the first state , the interior of the capillary needle is separated into two parts by the moving part, and when the moving part is in the second state, the two parts communicate; the negative pressure generating module communicates with the inside of the capillary needle when it is in the first state The space between the moving part and the first open end of the capillary needle makes the pressure in the space lower...

Embodiment 2

[0072] An application example of the single-cell mass spectrometry device and method according to Embodiment 1 of the present invention.

[0073] In this application example, the carrier has a matrix structure with rectangular grooves distributed in an array, and each groove is suitable for accommodating a single cell; the carrier is placed statically;

[0074] The capillary is set on the second mobile unit, such as a two-dimensional mobile platform, driven by the second mobile unit, so that the outlet end of the capillary is on the upper side of any selected cell; the inner diameter of the capillary is 3-6 μm, such as 3 μm , 5 μm or 6 μm, the inner diameter of the opening end is 1-3 μm, such as 1 μm, 2 μm or 3 μm, the energy of the outgoing light is 40-200 μJ, and the position where the outgoing light irradiates the capillary is 30-70 μm away from the opening end, so that from the capillary The outlet end of the laser tube forms a single droplet of skin level (1-600pL), and a...

Embodiment 3

[0094] The application example of the single-cell mass spectrometry device and method according to Embodiment 1 of the present invention is different from Embodiment 2 in that:

[0095] 1. The capillary is stationary; the second motor drives the capillary to rotate only in the vertical plane, so that the second open end corresponds to the inlet of the mass spectrometer;

[0096] The first moving unit is shared with the second moving unit, and drives the carrier to move two-dimensionally in the horizontal plane, so that the outlet end of the capillary is on the upper side of any selected cell, and with the cooperation of the second motor, the first movement of the capillary needle The open end faces the selected cell.

[0097] 2. The moving part is selectively in and out of the capillary needle driven by the first motor: the moving part is in the first state of the capillary needle: the inside of the capillary needle is separated into two parts, and moved out of the capillary n...

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Abstract

The invention provides a single-cell mass spectrometry device and method. The single-cell mass spectrometry device includes a carrier and a mass spectrometer. The carrier is used to carry a plurality of mutually separated cells. The mass spectrometer has a sample inlet; the transmission unit includes a capillary needle , a moving part, a negative pressure generating module, a driving module and a support; the two ends of the capillary needle are open and arranged on the support; the moving part is selectively in the first state and the second state; the negative pressure generating module communicates with the moving part in the capillary needle and the space between the first open end, so that the pressure in the space is lower than that outside the capillary needle; the drive module is used to rotate the capillary needle on the vertical plane, so that the second open end of the capillary needle corresponds to the injection port; the electrode is arranged on The second open end is suitable for connecting to a power supply; the first moving unit is used to drive the carrier or the transfer unit, so that the distance between the first open end of the capillary needle driven by the driving module and the cells on the carrier is 0-1mm. The invention has the advantages of high analysis efficiency and the like.

Description

technical field [0001] The invention relates to cell analysis, in particular to a single-cell mass spectrometry device and method. Background technique [0002] Cells are the basic units that make up living organisms. To understand the changes and behaviors of individual cells at various stages in a complex and changeable environment, methods for single-cell analysis are required. Mass spectrometry is a method for simultaneous analysis of multiple components. According to the different molecular weights of various components in cells, spectral peaks arranged according to mass numbers can be formed in mass spectrometry, and further through multi-stage mass spectrometry analysis, various components in cells can be obtained. molecular information. Since mass spectrometry does not require labels and does not need to know the information of the molecules to be tested in advance, it can quickly identify various unknown components in cells and obtain the omics information of prote...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/10G01N27/68G01N1/34G01N1/40
CPCG01N1/34G01N1/4055G01N15/1031G01N27/68G01N2001/4061G01N2015/1006
Inventor 闻路红甘剑勤刘云陈安琪毕磊洪欢欢李刚强
Owner CHINA INNOVATION INSTR CO LTD