High-temperature-resistant enterococcus faecalis and preparation method thereof

A technology of enterococcus faecalis and high temperature resistant type, which is applied in the field of high temperature resistant enterococcus faecalis and its preparation, can solve the problems of high price and difficult to use on a large scale, and can prevent diarrhea, promote intestinal peristalsis, and inhibit pathogenic bacteria. effect of growth

Inactive Publication Date: 2020-12-04
深圳市百科俊实业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, such products have entered the Chinese market from abroad, such as the microcapsules of Streptococcus faecalis "Cimeijian" in Switzerland, and "Pfaffia" in the Netherlands, etc., but the price is high and it is difficult to use them on a large scale.
In addition, the use of microecological preparations prepared by microencapsulation technology to produce compound feed for sea fish such as golden pomfret, and the application of this compound for golden pomfret breeding has rarely been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 100 parts of coco-based sucrose ester active agent, 90 parts of camellia oil ester, 60 parts of xylo-oligosaccharide, 50 parts of Bacillus licheniformis, 80 parts of faecalibacterium, and 40 parts of yeast.

[0028] Bacillus licheniformis 100 billion / gram, fecal enterobacteriaceae 20 billion / gram, yeast 10 billion / gram.

[0029] The production steps are:

[0030] (1) The selected strains were screened, rejuvenated, and improved by repeated gradual temperature rise to obtain probiotics with better high temperature resistance, and the selected strains were repeatedly bred by gradual temperature and mutation breeding. Raise the temperature and rejuvenate, and obtain Enterococcus faecalis with better high temperature resistance, which provides a strain guarantee for adding microecological preparations to the compound feed of sea fish such as golden pomfret;

[0031] (2) Add coco-based sucrose ester active agent, camellia oil ester and xylooligosaccharide to the heating bar...

Embodiment 2

[0037] 260 parts of coco-based sucrose ester active agent, 160 parts of camellia oil ester, 110 parts of xylooligosaccharide, 115 parts of Bacillus licheniformis, 110 parts of faecalibacterium, and 105 parts of yeast.

[0038] Bacillus licheniformis 210 billion / gram, fecal enterobacteria 45 billion / gram, yeast 11 billion / gram.

[0039] Preparation steps:

[0040] (1) The selected strains were screened, rejuvenated, and improved by repeated gradual temperature rise to obtain probiotics with better high temperature resistance, and the selected strains were repeatedly bred by gradual temperature and mutation breeding Raise the temperature and rejuvenate, and obtain Enterococcus faecalis with better high temperature resistance, which provides a strain guarantee for adding microecological preparations to the compound feed of sea fish such as golden pomfret;

[0041] (2) Add coco-based sucrose ester active agent, camellia oil ester and xylo-oligosaccharide into the heating barrel, ...

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Abstract

The invention relates to high-temperature-resistant enterococcus faecalis and a preparation method thereof. The formula of the high-temperature-resistant enterococcus faecalis consists of the following components in parts by weight of 100-400 parts of coconut oil based sucrose ester active agents, 90-200 parts of camellia oil esters, 60-160 parts of xylooligosaccharide, 50-150 parts of bacillus licheniformis, 80-120 parts of enterococcus faecalis and 40-180 parts of saccharomycetes. The high-temperature-resistant enterococcus faecalis is directly mixed in the processing course of a complete feed, after the high-temperature-resistant enterococcus faecalis and feed components are directly processed at high temperature, a large quantity of viable bacteria are reserved, the decline amplitude in the effective storage period is very small, the high-temperature-resistant enterococcus faecalis resists gastric acid, bile salt and other adverse chemical environments, can be quickly recovered andamplified after entering intestinal tracts of animals and being dissolved under the action of pancreatic fatty acid enzymes and the like, and can be subjected to colonization in the intestinal tracts, and the disease-resistant immunocompetence of the animals is greatly enhanced.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a high-temperature-resistant Enterococcus faecalis and a preparation method thereof. Background technique [0002] In response to the rapid development and expansion of the fishery industry and the increasingly serious problems of aquaculture diseases, fishery medicines play an irreplaceable role in my country's aquatic animal breeding and disease control. However, most of the current fishery medicines are imitation veterinary medicines, human medicines and pesticides, etc., which have problems such as lack of science, lack of pertinence, low efficacy, and great impact on the environment, which directly affect the effective prevention and control of aquatic diseases and the production of aquaculture products. quality and safety. Due to the intensification of farming and the disorderly expansion of the scale, the deterioration of the offshore farming environment, and the low ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/121A23K20/158A23K40/30A23K50/80
CPCA23K10/18A23K20/121A23K20/158A23K40/30A23K50/80
Inventor 叶明强谭玉强岳海林彭东亮龚哲宇
Owner 深圳市百科俊实业股份有限公司
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