Preparation method of metal organic framework nano vaccine without refrigerated storage

Abstract

The invention relates to a preparation method of a metal organic framework nano vaccine without refrigerated storage. The nano vaccine still keeps good biological activity when exposed to high-temperature, enzyme and long-term room temperature environments, the stability of the vaccine is remarkably improved, and the cold chain transportation problem of the vaccine is solved. The nano vaccine canenhance immune response, has a good adjuvant effect and can be used for preparing attenuated vaccines, protein vaccines, polysaccharide vaccines, nucleic acid vaccines or polypeptide vaccines. The nano vaccine takes polyethylene glycol as a mineralizer, the particle size is controllable, no organic reagent, microwave, high temperature or other severe conditions are involved in the process, the structures and activities of antigens and adjuvants cannot be damaged, the preparation process is simple, mature and stable, and large-scale production can be achieved.

Description

technical field [0001] The invention relates to a method for preparing a metal-organic framework nano-vaccine without refrigerated storage, and belongs to the technical field of biomedicine. Background technique [0002] Vaccination is an important means to prevent infectious diseases, it can effectively reduce the infection rate and mortality of various infectious diseases. However, many problems in the use process limit its wider application, such as ineffective against malaria, AIDS, tuberculosis and other diseases, cannot fully activate the immune system, and require cold chain (cold chain) transportation. Among them, the World Health Organization (WHO) has identified the problem of cold chain transportation as one of the most serious challenges facing vaccination. The annual cost of cold chain transportation for vaccines is about 200-300 million US dollars, accounting for about 80% of the entire vaccine project. This not only increases the cost of vaccines and the dif...

AI Technical Summary

Problems solved by technology

Nano-vaccine has made great progress in improving the immunogenicity of vaccines, but there are still some problems: (1) Low antigen load: although antigen load can be achieved by embedding, adsorption, coupling and other methods, but The preparation process is complex and cumbersome, and involves severe conditions such as oil-water interface, mechanical stirring, high temperature, and chemical reactions, which may destroy the structure and activity of the antigen, resulting in low antigen loading; (2) weak specific immune response, for example, the induction of antigen-specific Cytotoxic T-lymphocyte (CTL) response; (3) The use of toxic substances or organic solvents in the preparation process leads to poor biocompatibility or biodegradability

[0005] However, there are still some key problems that need to be solved urgently when MOF materials are used in vaccines: poor stability and easy degradation in the physiological environment in vivo; the process of loading antigens often involves severe conditions such as organic reagents, microwaves, and high temperatures, which are likely to affect the antigens. damage to structure and activity

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