Culture induction liquid for umbilical cord and placenta mesenchymal stem cells

Abstract

The invention discloses a culture induction liquid for umbilical cord and placenta mesenchymal stem cells. An alpha-MEM liquid culture medium is used as a solvent to dissolve the following substances:2-5 mmol / L of calcium chloride, 1-5 mmol / L of potassium chloride, 5-20 ng / mL of TGF-beta, 5-20 ng / mL of bFGF, 5-20 ng / mL of EGF, 5-20 ng / mL of IGF-1, 10-30 ng / mL of transferrin, 20-100 ng / mL of hesperidin, 5-50 ng / mL of selenious acid, 5-50 ng / mL of linoleic acid and 0.5-5 ng / mL of bovine serum albumin. The umbilical cord or placenta mesenchymal stem cells induced and cultured by the culture liquid can be used for skin transplantation, and can enhance the skin elasticity and improve the skin aging state.

Description

technical field [0001] The invention relates to a culture induction solution for umbilical cord and placenta mesenchymal stem cells. Background technique [0002] Now the skin is the largest organ in the human body, which has the functions of protection, excretion, temperature regulation and feeling of external stimuli, and is also the external manifestation of human aging. With the increase of age, a series of degenerative lesions will occur in the human skin, the skin becomes thinner, the elasticity fades, and wrinkles appear. [0003] There are many mechanism theories of skin aging, such as gene disorder theory, endocrine disorder theory, free radical theory, glycosylation, and immune decline theory. But no matter which theory, skin aging must be accompanied by the decline of elastic fiber content in the skin. [0004] Elastic fibers are mainly in the dermis of the skin, forming an elastic fiber network to maintain the elasticity of the skin. Mature elastic fibers are ...

AI Technical Summary

Problems solved by technology

Studies have also shown that transplanting mesenchymal stem cells in aging skin can improve some aging indicators of cells, increase serum and skin SOD activity, but the overall skin anti-aging effect is limited

One of the essential reasons is that mesenchymal stem cells still cannot secrete elastin in the body after transplantation, and the factors secreted after mesenchymal stem cell transplantation cannot promote the restoration of elastin secretion ability of recipient skin fibroblasts, because adult cells almost lost the ability to secrete elastin

Images