Application of pericarpium citri reticulatae essential oil to preparation of products for preventing or treating arthritis

A technology of tangerine peel essential oil and arthritis, applied in the field of medicine, can solve problems such as irritation, no research report, etc., and achieve the effects of reducing inflammatory factors, improving the degree of disease lesions, and alleviating joint tissue damage

Active Publication Date: 2020-12-15
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Tangerine peel essential oil is the main component of citrus essential oils. It has a mild stimulating effect on the gastrointestinal tract, can promote the secretion of digestive juices, and eliminate the accumulation of gas in the intestines. , asthma; in addition, it has certain antioxidant and anti-inflammatory activities, but the application in the preparation of anti-arthritis drugs has not been reported

Method used

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  • Application of pericarpium citri reticulatae essential oil to preparation of products for preventing or treating arthritis
  • Application of pericarpium citri reticulatae essential oil to preparation of products for preventing or treating arthritis
  • Application of pericarpium citri reticulatae essential oil to preparation of products for preventing or treating arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The improvement situation of embodiment 1 tangerine peel essential oil to the rat rheumatoid arthritis induced by CFA

[0037] 1. Experimental method

[0038] (1) Animal grouping:

[0039] Before the experiment, 42 adult male rats were taken and adaptively fed for one week (7 days). Forty-two rats were randomly divided into seven groups of six, and the skin of the right toe was routinely disinfected before arthritis developed. Except for the normal control group (Con), rats in other groups were subcutaneously injected with 0.1 mL of CFA on day 8 and day 16 for arthritis induction.

[0040] (a) Normal control group (Con: normal control): no experiment was conducted on it, and it was kept from day 0 to the end of the experiment (day 21).

[0041](b) Negative control group (NC: negative control group): From the 8th day until the end of the experiment (21st day), Tween 80 was administered according to the weight of 1 mL / kg, and poured into the stomach through a syringe. ...

Embodiment 2

[0058] Example 2 Effect of tangerine peel essential oil on CFA-induced injury of rat joint tissue mucosal cells

[0059] 1. Experimental method

[0060] The experimental group and dosing regimen of this example are the same as in Example 1. After the administration of the rats, the rats were killed by vertebral dislocation, and the complete joint tissues were taken out to make paraffin sections and stained with HE. The operation steps included:

[0061] Soak the joint tissue in 4% paraformaldehyde solution for 6 hours;

[0062] Dehydration: Gradually increase from low concentration alcohol to high concentration alcohol, put in 50%, 70%, 80%, 95% alcohol for 12-24 hours each, 100% alcohol for 2-3 hours, then put xylene and half an hour in alcohol, then put in xylene until the specimen is transparent;

[0063] Wax penetration: move the joint tissue into xylene plus paraffin, put it in a 60°C incubator for half an hour, and then move it into the paraffin to allow the paraffin t...

Embodiment 3

[0073] Example 3 The effect of tangerine peel essential oil on the inflammatory response in rat joint tissue induced by CFA

[0074] 1. Experimental method

[0075] The experimental grouping and dosing scheme of this example are the same as in Example 1. After the administration of the rats, the rats were killed by spinal dislocation, and the complete joint tissues were taken out to make paraffin immunohistochemical sections. The operation steps included fixation:

[0076] Dewaxing and Hydration:

[0077] 1. Immerse the slices in xylene I10min and xylene I (10min) in turn.

[0078] 2. Immerse again in absolute ethanol I (5min) absolute ethanol II (5min)-95% alcohol (5min)-80% alcohol (5min)-70% alcohol (5min), then rinse with deionized water twice, each time 2min.

[0079] Antigen retrieval:

[0080] 3. Put the tissue slice into the repair box, and then add an appropriate amount of 0.01M sodium citrate buffer (pH6.0) or EDTA repair solution (pH9.0), and the liquid surface ...

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Abstract

The invention provides an application of pericarpium citri reticulatae essential oil to preparation of products for preventing or treating arthritis. Research results show that a pericarpium citri reticulatae essential oil component can relieve CFA-induced rheumatoid arthritis of rats and can effectively relieve joint tissue damage caused by CFA; and the pericarpium citri reticulatae essential oilcan inhibit the lesion degree of arthritis by inhibiting activity expression of tumor necrosis factors TNF-alpha and COX-2, and control the joint arthritis reaction, so that the pain of a patient caused by the arthritis is relieved to the greatest extent, and a new choice is provided for developing novel anti-arthritis medicines.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of tangerine peel essential oil in the preparation of products for preventing or treating arthritis. Background technique [0002] Arthritis is a global chronic inflammatory joint disease. Arthritis generally refers to an inflammatory disease that occurs in one or more joints of the human body, mainly manifested by swelling and tenderness, and is caused by inflammatory invasion in the joint cavity, cartilage or bone degeneration, trauma or other factors, and can be divided into Dozens of them. The clinical manifestations are joint redness, swelling, heat, pain, dysfunction and joint deformity. In severe cases, it will lead to joint disability and affect the quality of life of patients. [0003] There are currently 355 million arthritis patients worldwide. In Asia, one in six people will suffer from arthritis at some point in their lives, and it is the number o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A61P19/02A61P29/00
CPCA61K36/752A61P19/02A61P29/00
Inventor 张蓝月李春莲朱海枰潘春杏徐雅妮石耀辉向洪平
Owner GUANGDONG UNIV OF TECH
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