A method for identifying tobacco red star disease by artificial inoculation

A tobacco red spot disease and artificial inoculation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, and biological material sampling methods, can solve the problem of unsatisfactory onset, no disease after inoculation, and large differences in the incidence of red spot disease Major problems, to achieve the effect of short onset period, moderate onset degree, accurate and reliable results

Active Publication Date: 2022-05-10
TOBACCO RES INST CHIN AGRI SCI ACAD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a method for identifying tobacco red spot disease by manual inoculation, which solves the problem that when several existing methods are used to inoculate red spot disease bacteria, the impact on the incidence of red spot disease is very different, and the emergence of The problem of inoculation without disease or unsatisfactory disease

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  • A method for identifying tobacco red star disease by artificial inoculation
  • A method for identifying tobacco red star disease by artificial inoculation
  • A method for identifying tobacco red star disease by artificial inoculation

Examples

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no. 1 example

[0043] see Figure 1-5, the embodiment of the present invention provides a technical solution: a method for identifying tobacco red spot disease artificial inoculation, comprising the following methods:

[0044] S1: Cultivation of Alternaria Alternaria: Take the bacteria cake of the activated test strain, transfer it to a PDA plate, and culture it at a constant temperature of 28°C for 5d, 10d, and 15d respectively, and set aside;

[0045] S2: Preparation of inoculum: prepare mycelium block inoculum, mycelia inoculum, and spore liquid inoculum, and the concentrations of the spore liquid are 105, 106, and 107 spores / mL respectively;

[0046] S3: Leaf wound injection: includes the following steps:

[0047] T1: Select the leaves at the lower part of the tobacco plant, wipe them with 75% alcohol cotton balls and wash them with sterile water, put them in a clean seedling tray, take 6-10 inoculation points on each leaf, and pierce the leaves at the inoculation points with the needle...

no. 2 example

[0110] Please refer to Figure 6 , Figure 7 and Figure 8 , Based on the identification method for manual inoculation of tobacco red spot disease provided in the first embodiment of the present application, the second embodiment of the application proposes another method for identification of manual inoculation of tobacco red spot disease. The second embodiment is only a preferred mode of the first embodiment, and the implementation of the second embodiment will not affect the independent implementation of the first embodiment.

[0111] Specifically, the difference of the manual inoculation identification method for tobacco red spot disease provided by the second embodiment of the present application is that the manual identification method for tobacco red spot disease also includes a blade inoculator, the blade inoculator mounting plate 1, the One side of the mounting plate 1 is provided with a clamping assembly 2, the front of the mounting plate 1 and one side of the clam...

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Abstract

The invention discloses an identification method for artificial inoculation of tobacco red spot disease, which is characterized in that it comprises the following methods: S1: Cultivation of red spot disease bacteria: take the activated bacterial strains to be tested and transfer them to PDA plates, and place them at 28°C respectively. Constant temperature culture for 5d, 10d, 15d, standby; S2: Preparation of inoculum: prepare mycelium block inoculum, mycelia inoculum, spore liquid inoculum, the concentration of spore liquid is 105, 106, 107 spores / mL concentration respectively; involving Tobacco brown spot identification technology field. In the manual inoculation identification method for tobacco red star disease, when the leaves are inoculated, when the push handle of the syringe is pressed, the lifting ear can be pulled upward by fingers, the lifting ear can push the slider upward, the slider moves upward and drives the rotating rod to push the connecting block, and connects The block pushes the arc-shaped stopper to clamp the surface of the syringe to prevent the syringe from moving relative to the arc-shaped stopper when the push handle is pushed.

Description

technical field [0001] The invention relates to the technical field of identification of tobacco red spot disease, in particular to an identification method for manual inoculation of tobacco red spot disease. Background technique [0002] Tobacco red spot disease is a fungal disease of leaves caused by Alternaria, which belongs to the subphylum Deuteromycota. The disease occurs in the mature stage of tobacco leaves, starting from the lower leaves of the tobacco plant. As the leaves mature, the disease spots gradually develop from bottom to top. The spots are roughly circular with necrotic centers and concentric rings, usually surrounded by a yellow halo, and the spots vary in size from small lentigos (approximately 1 mm) to large lesions 1 to 3 cm in diameter. The disease has the characteristics of short incubation period and rapid epidemic speed. Under suitable environmental conditions, it can cause a large epidemic in a short period of time and bring huge losses to tobacc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12M1/26C12R1/645
CPCC12N1/14C12M33/04
Inventor 王杰雷庭王静罗倩茜丁忠林舒芳靖姚峰喻延苟正贵何孝磊
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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