Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for quantitatively analyzing content of sorbose in fermentation broth

A quantitative analysis, sorbose technology, applied in the direction of analyzing materials, material analysis by optical means, measuring devices, etc., can solve the problems of long time consumption and complicated operation process, and achieve short time consumption, simple operation process and high detection efficiency. Effect

Active Publication Date: 2020-12-15
山东金璋隆祥智能科技有限责任公司
View PDF14 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the invention provides a method for quantitative analysis of sorbose content in fermented liquid, which solves the problem that the detection methods mainly used in present sorbose fermented liquid are titration, high performance liquid chromatography, etc.; Toxic and harmful chemical reagents are used, and the operation process of the above two methods is relatively complicated and takes a long time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quantitatively analyzing content of sorbose in fermentation broth
  • Method for quantitatively analyzing content of sorbose in fermentation broth
  • Method for quantitatively analyzing content of sorbose in fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Such as Figure 1-2 As shown, the embodiment of the present invention provides a method for quantitative analysis of sorbose content in fermented liquid, comprising the following specific steps:

[0028] S1. First establish the orthogonal signal spectrum preprocessing algorithm, the algorithm process is as follows: first standardize the original calibration set spectral matrix X and the information matrix Y to be measured;

[0029] S2. Calculate M=I-X'Y(Y'XX'Y) -1 Y'X, I is the identity matrix;

[0030] S3. Calculate Z=X;

[0031] S4. Perform singular value decomposition on ZZ', and extract the first p eigenvalues ​​g that require orthogonal processing i and the corresponding eigenvector C i ;

[0032] S5. Calculate the weight vector w i =MX'C i g i ;

[0033] S6. Calculate the score vector t i =C i g i ;

[0034] S7. Calculate load vector p i =X't i (t i 't i );

[0035] S8. Subtract the quadrature signal from X: X 0x =X-∑t i p' i ;

[0036] S9. F...

Embodiment 2

[0040] In this embodiment, on the basis of Embodiment 1, a group of experiments are carried out, and the experimental steps are as follows:

[0041]1. Sample collection: Take samples of the 8-hour fermentation time between the 12th hour and the 20th hour of the fermentation process. The first 30 samples are taken every 10 minutes, and the last 30 samples are taken every 5 minutes. The total sampling 60, packed in glass test tubes, placed in the freezer after scanning the spectrum. Spectrum collection: put the fermentation broth into a sample cup, and use a GSA103 near-infrared spectrometer for spectrum collection. Randomly select 50 samples from the spectra of 60 samples for modeling, and the remaining 10 samples will not participate in the modeling as external verification samples to verify the accuracy of the model;

[0042] 2. Collect the original spectrum of the sample and preprocess it with the orthogonal signal correction algorithm to obtain the following: figure 1 Pre...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for quantitatively analyzing the content of sorbose in fermentation broth, and relates to the technical field of quantitative analysis of the content of sorbose. The method for quantitatively analyzing the content of sorbose in the fermentation liquor comprises the specific steps of S1, firstly, establishing an orthogonal signal spectrum preprocessing algorithm which comprises the step of firstly, standardizing an original calibration set spectrum matrix X and a to-be-detected information matrix Y; S2, calculating M=I-X'Y(Y'XX'Y)<-1>Y'X, wherein I is a unit matrix; S3, calculating Z=X; S4, performing singular value decomposition on the ZZ', and extracting the first p feature values gi needing orthogonal processing and the corresponding feature vectors Ci; S5, calculating a weight vector wi=MX'Cigi; and S6, calculating a score vector ti=Cigi. According to the method, the near infrared spectrum of the sorbose content in the fermentation broth is preprocessed by using an orthogonal signal correction method, and then the correction model is established by using a local weight regression method, so that the sorbose content in the fermentation broth can becompletely measured and analyzed, the working efficiency is greatly improved, and the method is worthy of vigorous popularization.

Description

technical field [0001] The invention relates to the technical field of quantitative analysis of sorbose content, in particular to a method for quantitative analysis of sorbose content in fermentation broth. Background technique [0002] Vitamins are essential elements for human life activities, mainly in the form of coenzymes or prosthetic groups to participate in various chemical reactions of organisms, vitamins play an important role in medical treatment, such as vitamin B family for the treatment of neuritis, keratitis and other inflammations , Vitamin D is an important drug for treating rickets, etc. Vitamins are also used in animal husbandry and feed industry. The production of vitamins mostly uses chemical synthesis. Later, it was discovered that some microorganisms can complete some important steps in vitamin synthesis. ; On this basis, the semi-synthetic method combining chemical synthesis and biotransformation has been widely used in vitamin production. At present, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G16C20/20G16C20/70G01N21/359G01N21/3577G06F17/16
CPCG16C20/20G16C20/70G01N21/359G01N21/3577G06F17/16
Inventor 邹振民孙茂耿龙飞朱传港
Owner 山东金璋隆祥智能科技有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com