MIA primer, probe and kit for detecting COVID-19 and application of MIA primer and probe

A pneumonia virus and kit technology, which is applied in the field of kits and applications, probes, and MIA primers for the detection of new coronary pneumonia virus, can solve the problems of invisible carriers, such as inability to detect antibodies, diagnostic screening, complexity, and easy aerosol pollution. Achieve good market application prospects, simplify system design, and avoid inaccuracies
CN112094947APending Publication Date: 2020-12-18HANGZHOU BAOLIN BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HANGZHOU BAOLIN BIOTECHNOLOGY CO LTD
Publication Date
2020-12-18

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Abstract

The invention discloses an MIA primer, a probe and a kit for detecting COVID-19 and an application of the MIA primer and the probe. According to the invention, the MIA primer and the probe are respectively designed based on a nucleocapsid N gene, an envelope E gene and an ORF1ab region of the COVID-19 publicized by NCBI. According to the MIA method disclosed by the invention, the detection of ORF1ab, E and N target genes of COVID-19 is realized in 3-15 minutes for the first time, and the detection sensitivity reaches 100 copies / ml (sample template concentration). The MIA method has no non-specific cross reaction with pathogen nucleic acid samples such as type A influenza, type B influenza and respiratory syncytial virus.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to MIA primers, probes, kits and applications for detecting novel coronavirus pneumonia. Background technique

[0002] Corona Virus Disease 2019 (COVID-19), referred to as "New Coronary Pneumonia", named by the World Health Organization as "2019 Coronavirus Disease", refers to the pneumonia caused by 2019 Novel Coronavirus infection. The clinical manifestations of pneumonia patients infected by the new coronavirus are: fever, fatigue, dry cough as the main manifestations, nasal congestion, runny nose and other upper respiratory symptoms are rare, and hypoxic and hypoxic states will occur. About half of the patients developed dyspnea more than a week later, and severe cases could rapidly progress to acute respiratory distress syndrome, septic shock, difficult-to-correct metabolic acidosis, and coagulation dysfunction. According to the "New Coronavirus Pneumonia Diagnosis and...

Claims

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