A kind of nano-disinfection coating for disinfection of livestock and poultry breeding places and its application
A technology for livestock and poultry breeding and places, which is applied in applications, disinfectants, alginic acid coatings, etc., can solve the problems of short adhesion time of liquid disinfectants and ineffective killing effect, and achieve long-lasting broad-spectrum sterilization and virus killing effect, fast and efficient killing effect, and effect of remarkable bactericidal effect
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Embodiment 1
[0022] A nano-disinfected coating with a livestock and poultry farm, consists of the following components:
[0023]
[0024] The above nano-disinfected coating is diluted with water 10 to 20 times, and the dose of 100 ml / m2 is sprayed in the culture dish (57cm) 2 ), E. coli is then seeded (G - Fungus), inverted culture for 14 hours, such as figure 1 As shown: The nano-disinfectous coating of the present invention has a significant sterilization inhibitory effect on E. coli.
Embodiment 2
[0026]
[0027] The above nano-disinfected coating is diluted with water 10 to 20 times, and the dose of 100 ml / m2 is sprayed in the culture dish (57cm) 2 Inoculated inoculated with Streptococcus, then + Fungus), inverted culture for 14 hours, such as figure 2 As shown: The negative control group did not add a nanofin coating, and the number of colonies in the two petri dishes were 70 and 51, respectively, and the experimental group of the nano-disinfectant coating of the present invention was added, and the colonies in the four petri dishes were 13 respectively. 13 , 18, 9 and 6, its bactericidal rate is 81%. The nanofinishing coating of the present invention has a significant sterilization of bacteriobacteria in Streptococcus.
Embodiment 3
[0029] Nano-disinfectant coating is diluted 20 times in Example 1, and the nano-disinfection coating is diluted with a pig pseudo-rabies (PRV: DNA virus, a capsule film) mixed under 37 ° C for different times (15 min, 30 min, 1 h), infected PK15 cell line, after 3 days, the virus liquid was harvested, and the fluorescence quantitative PCR was detected. As shown in Table 1, the product was 99.94% to PRV at around 1 h, which has a significant killing viral effect. NC is a negative control group, and the cells were only incorporated into normal medium, no virus and nanofin coating; PC was a positive control group, and the cells were added to the virus liquid in normal medium, and did not add nano-disinfected coating; X group The virus was added to the nano-disinfectous coating mixture in normal medium.
[0030] Table 1 PRV fluorescent quantitative detection results
[0031]
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