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Environmental stress-resistant strain XN-K13 and application thereof

A XN-K13, environment-resistant technology, applied in the direction of application, bacteria, chemicals for biological control, etc., can solve the problem of lower utilization rate, achieve the effect of promoting root dry weight and improving the ability to withstand environmental stress

Active Publication Date: 2020-12-29
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Chemical fertilizers play a huge role in modern agricultural production, but with the increase in the use of chemical fertilizers, their utilization rate decreases year by year

Method used

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  • Environmental stress-resistant strain XN-K13 and application thereof
  • Environmental stress-resistant strain XN-K13 and application thereof
  • Environmental stress-resistant strain XN-K13 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Isolation and identification of strain XN-K13 resistant to environmental stress

[0040] 1.1 Isolation of strain XN-K13 resistant to environmental stress

[0041] The isolation of the environmental stress-resistant strain XN-K13 includes three steps: sampling, enrichment, primary screening, and secondary screening, as follows:

[0042] 1.1.1 Sampling

[0043] Samples were taken from the soil of Guangxi Medicinal Botanical Garden (E108°19', N22°51'), put into clean sampling bags, marked, and stored in a refrigerator at 4°C as rhizosphere soil samples.

[0044] 1.1.2 Enrichment and primary screening

[0045] Weigh 1.0 g of rhizosphere soil sample, put it into a test tube filled with 9 mL of sterile deionized water, shake and mix thoroughly, 37 ° C, 200 r / min, shake and cultivate for 30 min, and dilute it in a ten-step gradient to obtain a dilution; To isolate salt-tolerant bacteria, add NaCl with a mass concentration of 10g / L as salt stress, draw 100uL of the diluted sol...

Embodiment 2

[0064] Accurate characterization of potassium solution by environmental stress-resistant strain XN-K13:

[0065] The dissolving ability of Enterobacterludwigii XN-K13 to poorly soluble potassium was preliminarily evaluated by the improved selection medium for potassium-solubilizing bacteria: use a sterile toothpick to puncture the isolated and purified strain obtained in 1.1. Potassium feldspar was used as the insoluble potassium source in the center of the improved selective medium for potassium-dissolving bacteria, placed in a 37°C incubator and cultured for 5 days, each group was treated three times, and the potassium-dissolving circle was observed to measure the colony diameter (d) and the potassium-dissolving circle diameter ( D), the results are as shown in table 2, calculate the ratio of solution potassium circle and bacterium colony diameter ( Figure 4 ); from Table 2 and Figure 4 It can be concluded that the ratio of the potassium-dissolving circle to the colony di...

Embodiment 3

[0069] Quantitative analysis of potassium solution by environmental stress-resistant strain XN-K13:

[0070] Potassium standard solution: Accurately weigh 1.9068g of analytically pure KCl that has been dried (dried at 105°C for 4-6 hours), dissolve it in 200mL of deionized water, and set the volume to 1L. Shake well to obtain 1000mg / L of potassium. Put 25mL of the solution into a 250mL volumetric flask to constant volume and shake well, then it will be a potassium standard solution containing 100mg / L of potassium.

[0071] 3% sodium tetraphenylborate solution: Weigh 3g sodium tetraphenylborate and dissolve it in 100mL deionized water, add 10 drops of 0.2mol / L sodium hydroxide solution to adjust the pH to 8-9, let it stand overnight, and filter it with dense filter paper Filter and store in a brown bottle.

[0072] 0.05mol / L borax solution: Weigh 19.07g borax and dissolve it in 1000mL deionized water.

[0073] Formaldehyde-EDTA solution: Weigh 2.5g EDTA disodium salt and diss...

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Abstract

The invention discloses an environmental stress-resistant strain XN-K13, which is classified and named as Enterobacter ludwigii XN-K13, is preserved in China Center for Type Culture Collection on May25, 2020 at Luojia Mountain of Wuhan City, Hubei Province, China, and has a preservation number of CCTCC NO: M2020145. The environmental stress-resistant strain XN-K13 provided by the invention can resist potassium deficiency, resist high salt and alkali, resist heavy metals and drought environmental stress, and the environmental stress-resistant strain XN-K13 can also improve the environmental stress resistance of plants. The environmental stress-resistant strain XN-K13 has a remarkable promoting effect on the seed germination rate, fibrous root number, bud length, radicle length, hypocotyledonary axis length, seedling dry weight and root dry weight of corn seeds and seedlings under salt stress, cadmium (Cd) stress and drought stress.

Description

technical field [0001] The invention belongs to the technical field of plant growth-promoting bacteria, and specifically relates to an environmental stress-resistant bacterial strain XN-K13 with high efficiency and resistance to salt and insoluble potassium and its application. Background technique [0002] Chemical fertilizers play a huge role in modern agricultural production, but with the increase in the use of chemical fertilizers, their utilization rate decreases year by year. Residues of pesticides and chemical fertilizers release a large amount of harmful substances to the environment, pollute soil, water sources and food, and pose a great threat to human health and living environment. The call for protecting the ecological environment and producing safe food is becoming stronger and stronger. Therefore, the development and utilization of new fertilizer sources that replace chemical fertilizers to meet the needs of the development of green agriculture and green food h...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P21/00C05F11/08C12R1/01
CPCA01N63/20C05F11/08C12R2001/01C12N1/205
Inventor 李有志樊宪伟黄巧
Owner GUANGXI UNIV
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