x-str fluorescence amplification system, kit and application

Abstract

The invention discloses an X-STR fluorescent amplification system, kit and application. The amplification system includes 29 pairs of primers corresponding to 28 X-STR sites and one Amel site on the X chromosome. The nucleotide The sequence is shown in SEQ ID NO.1‑58. The 28 STR sites in the X chromosome corresponding to the amplification system designed in the present invention include linkage groups of multiple X chromosomes, not only the number of detection sites is large, but also distributed in the short arm of the X chromosome, centromere Points and long arms, so it can effectively improve the detection accuracy, especially to better meet the high requirements of public security and judicial organs for X-STR detection applications.

Description

technical field

[0001] The invention belongs to the technical field of biological detection, and in particular relates to an X-STR fluorescence amplification system, a kit and an application. Background technique

[0002] Short tandem repeats (Short tandem repeats, STR) are currently the mainstream genetic markers used in forensic DNA typing research. Human genome STR (short tandem repeat sequence) is a DNA genetic marker formed by tandem repeats with a few bases as the core unit. The distinction between different races, different populations and even different individuals is through the difference in core unit sequence and repeat number, which also constitutes the genetic polymorphism of STR. In the genome, there is an STR site every 15-20kb on average, accounting for 10% of the genome, mostly in non-coding regions and introns, the repeat unit is 2-6bp, the number of repeats is 10-60 times, and the fragment size In 70 ~ 500bp, and was co-dominant inheritance according to ...

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