Non-small cell lung cancer circulating tumor cell separation system and method

Abstract

The invention relates to a non-small cell lung cancer circulating tumor cell sorting system and method. The non-small cell lung cancer circulating tumor cell sorting system comprises micro-column sorting chips, an oscillatory flow system and a flow cytometry counting functional area, wherein the micro-column sorting chips are used for sorting non-small cell lung cancer circulating tumor cells; theoscillatory flow system is used for providing bidirectional flow field force for the non-small cell lung cancer circulating tumor cells and relieving the blockage of the non-small cell lung cancer circulating tumor cells among the micro-column sorting chips; and the flow cytometry counting functional area is used for counting the sample feeding number and the sample discharging number of the non-small cell lung cancer circulating tumor cells and evaluating the sorting result of the non-small cell lung cancer circulating tumor cells. Through the shape, spacing and multilayer arrangement setting of array columns, automatic sorting of the non-small cell lung cancer circulating tumor cells is realized in cooperation with driving of oscillatory flow, and in addition, no biochemical marker exists in the sorting process; the cell damage is small; and the problems of cell blockage in the sorting chips in the process and the influence of bubbles on the sorting of the non-small cell lung cancercirculating tumor cells can be solved.

Description

technical field [0001] The present disclosure relates to the field of separation and enrichment of tumor cells in blood, in particular to a system and method for sorting non-small cell lung cancer circulating tumor cells. Background technique [0002] Circulating tumor cells (CTCs) refer to tumor cells that have acquired the ability to break away from the basement membrane from primary tumors or metastatic tumors and invade into blood vessels or lymphatic vessels through the tissue matrix, and circulate in the body with blood. . CTCs are likely to carry key information such as the location, environment, and development of tumors in situ or metastases, and are of great significance in early detection, cancer metastasis, formulation of personalized treatment plans, and prognosis assessment. However, due to the inherent properties of CTCs such as extreme rarity, high heterogeneity, and phenotypic diversity, it has brought great challenges to the isolation and enrichment of CTC...

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Problems solved by technology

[0008] In view of this, the present disclosure proposes a non-small cell lung cancer circulating tumor cell sorting system and method, which can realize automatic sorting of non-small cell lung cancer circulating tumor cells, and the sorting process has no biochemical markers, and the cell damage is small, and Solve the problem of the influence of cell blockage and air bubbles in the sorting chip on the sorting of non-small cell lung cancer circulating tumor cells

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