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A kind of method of alfalfa-Bacillus joint remediation of heavy metal polluted soil

A technology for bacillus and polluted soil, applied in the field of polluted soil remediation, can solve problems such as unfavorable cadmium removal, reduction of soil microbial diversity, impact on soil micro-ecological environment, etc., to achieve resource utilization, low operating cost, and improved removal effect. Effect

Active Publication Date: 2022-04-08
ECOLOGY INST SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is not conducive to the removal of cadmium in the soil by cutting the above-ground part of alfalfa in multiple crops.
[0007] The Chinese literature "The effect of different phytoremediation of heavy metal compound polluted soil on the number of microorganisms and enzyme activities in the soil" (Chen Haiyan, Fan Ting, etc., "Environmental Protection", 2018) This document uses alfalfa as a remediation plant to conduct experiments on contaminated soil in mining areas , have studied the impact of alfalfa on microbial quantity and enzyme activity in heavy metal (Cu, Zn, Pb, Cd) compound polluted soil, the microbial quantity in the soil of planting alfalfa increases, and soil microbial diversity index significantly improves; But the present invention finds As a restoration plant, alfalfa was tested in cadmium-contaminated soil instead of heavy metal-contaminated soil. The experimental results showed that the number of microorganisms in the soil planted with alfalfa decreased, and the soil microbial diversity decreased significantly. Although long-term planting can reduce Cadmium pollution in soil, but due to the reduction of microbial quantity and diversity will seriously affect the soil micro-ecological environment

Method used

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  • A kind of method of alfalfa-Bacillus joint remediation of heavy metal polluted soil
  • A kind of method of alfalfa-Bacillus joint remediation of heavy metal polluted soil
  • A kind of method of alfalfa-Bacillus joint remediation of heavy metal polluted soil

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Experimental program
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Effect test

Embodiment 1

[0031] A method for joint remediation of heavy metal-contaminated soil by Medicago-Bacillus.

[0032] Preparation of cadmium-loaded soil: take the surface soil of the vegetable field in the east area of ​​Shandong Academy of Sciences, air-dry it naturally, pass it through a 2mm sieve, add cadmium chloride solution according to the water-to-soil ratio of 1mL:1.2g, and balance for 60 days. The final total cadmium concentration in the soil is 3.8mg / kg.

[0033] Preparation of agricultural waste matrix: corn stalks were crushed into 1-3 cm, watered to keep the moisture content of the matrix at 70% by mass fraction, and aerobically fermented for 40 days.

[0034] Bacillus subtilis TTL1 was obtained from the Microorganism Preservation Bank of the Institute of Ecology, Shandong Academy of Sciences, which can be purchased here, and the preservation number is Z206D4.

[0035] Bacillus subtilis BsG2, from the China Agricultural Microorganism Culture Collection Management Center, can b...

Embodiment 2

[0050] A method for joint remediation of heavy metal-contaminated soil by Medicago-Bacillus.

[0051] Preparation of cadmium-loaded soil: take the surface soil of the vegetable field in the east area of ​​Shandong Academy of Sciences, air-dry it naturally, pass through a 2mm sieve, add cadmium chloride solution according to the water-to-soil ratio of 1mL:1.5g, and balance for 60 days. The final total cadmium concentration in the soil is 5.5mg / kg.

[0052] Preparation of agricultural waste matrix: crush wheat straw into 1-3 cm, water to keep the mass moisture content at 65%, and aerobically ferment for 35 days.

[0053] Preparation of Bacillus subtilis BsG2 cell suspension: Luria-Bertani liquid medium was sterilized at 121°C for 30 minutes, added with a volume ratio of 1% Bacillus liquid strain, activated at 35°C and 150rpm for 2 days, and then The bacterial solution was inserted into the agricultural waste matrix after autoclaving according to the solid-liquid ratio of 1g:10...

Embodiment 3

[0065] A method for combined remediation and improvement of heavy metal-contaminated soil by Medicago-Bacillus.

[0066] Preparation of cadmium-loaded soil: take the surface soil of the vegetable field in the east area of ​​Shandong Academy of Sciences, air-dry it naturally, pass it through a 2mm sieve, add cadmium nitrate solution according to the water-to-soil ratio of 1mL:2g, and balance for 60 days. The final total cadmium concentration in the soil is 4.0mg / kg .

[0067] Preparation of Bacillus subtilis BGL3 cell suspension: Luria-Bertani liquid culture medium was sterilized at 121°C for 30 minutes, added with a volume ratio of 1% Bacillus liquid strain, activated at 35°C and 150rpm for 2 days, and then The bacterium solution was added to the Luria-Bertani liquid medium after high-pressure steam sterilization at a volume ratio of 1%, and expanded at 35° C. and 150 rpm for 7 days.

[0068] The final fermentation broth of the bacillus thalline is in a liquid state, wherein ...

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Abstract

The invention relates to a method for combined restoration of heavy metal-contaminated soil by alfalfa-bacillus. The heavy metal-contaminated soil is mainly sown with alfalfa seeds after the heavy metal-contaminated soil is readjusted. After the alfalfa seeds germinate, the rhizosphere soil is inoculated with Bacillus bacterial agent, and the alfalfa grows 90 to 120 Days later, harvest the aboveground part of the plant, concentrate it on disposal, leave the underground part in the soil, and then carry out a new round of alfalfa sowing and bacillus inoculation until the heavy metal content in the polluted soil meets the soil environmental quality standard; the present invention combines alfalfa-bacillus Remediation of heavy metal-contaminated soil avoids soil micro-ecological environment damage caused by long-term planting of alfalfa in cadmium-contaminated soil; it can effectively transfer heavy metals from the underground part of alfalfa to the above-ground part by cutting the above-ground part of alfalfa in multiple crops. The removal of cadmium in the soil saves the cost of planting.

Description

technical field [0001] The invention belongs to the field of contaminated soil remediation, and in particular relates to a method for jointly remediating heavy metal-contaminated soil with alfalfa and bacillus Background technique [0002] Soil heavy metal pollution has become an environmental problem that seriously restricts agricultural development and endangers the safety of agricultural products. Traditional soil heavy metal pollution remediation technologies mainly include guest soil method, passivation method, electrokinetic method, leaching method, plant and microbial remediation method. [0003] Phytoremediation technology has low cost and good ecological benefits, but the slow growth of remediation plants, low biomass, poor tolerance, and small accumulation of heavy metals limit its large-scale application and promotion. Alfalfa is a perennial legume with rich nutrition, nitrogen fixation function and landscape value. It can also be used as a restoration plant to a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10
CPCB09C1/105B09C2101/00
Inventor 李琪邢颖娜张强王加宁陈贯虹傅晓文季蕾李天元
Owner ECOLOGY INST SHANDONG ACAD OF SCI