Image registration method, device and computer program product

Abstract

The invention discloses an image registration method, device and a computer program product. The image registration method comprises the steps of performing first registration on a to-be-registered image based on a reference image, including determining a predetermined area on the to-be-registered image and a first offset of a corresponding predetermined area on the reference image, and moving allbright spots on the to-be-registered image based on the first offset; and performing second registration on the to-be-registered image after the first registration based on the reference image, including combining the to-be-registered image after the first registration with the reference image, and calculating offsets of all overlapped bright spots in a predetermined area on the combined image todetermine a second offset, wherein two or more bright spots of which the distance is smaller than a predetermined pixel are one overlapped bright spot; and moving all bright spots on the to-be-registered image after the first registration based on the second offset so as to realize registration of the to-be-registered image. According to the method, high-precision image registration can be realized, and the situation of high-precision requirement on image correction is met.

Description

technical field [0001] The present invention relates to the field of image processing, in particular to an image registration method, an image registration device and a computer program product with an image registration function. Background technique [0002] In applications that include images of the same object or objects captured multiple times at different times, it is generally necessary to perform deflection correction / registration on the obtained multiple images, so that the change information of the object can be accurately obtained based on the deflection-corrected images. [0003] In a platform that uses images of nucleic acid molecules for nucleic acid sequence determination, it is generally necessary to collect images of nucleic acid molecules in the same field of view at different times on mobile hardware. information, the sequence information of the nucleic acid molecules in the field of view can be determined. In the actual image acquisition, due to the cert...

AI Technical Summary

Problems solved by technology

In the actual image acquisition, due to the certain precision of hardware movement, that is, there is a certain error between the specified movement amount and the actual movement amount, and / or changes in the shape of nucleic acid molecules caused by changes in the environment / system where the nucleic acid molecule is located, The position information of the immobilized nucleic acid molecule in the image of the field of view obtained at multiple moments will be different, making it difficult to directly use the obtained image information to accurately identify and determine the sequence of the nucleic acid molecule

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