Fortunella margarita extract as well as preparation method and application thereof

A technology of extract and kumquat, which is applied in the field of kumquat extract and its preparation, can solve the problems of target organ damage and side effects that cannot be ignored, and achieve the effects of no toxic side effects, heart protection, and easy access to resources

Active Publication Date: 2021-02-02
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the treatment of hypertension is a long-term process, and the side effects of target organ damage cannot be ignored.

Method used

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  • Fortunella margarita extract as well as preparation method and application thereof
  • Fortunella margarita extract as well as preparation method and application thereof
  • Fortunella margarita extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 50% ethanol preparation of kumquat extract

[0026] Kumquat: purchased from the Suichuan planting base in Jiangxi Province, identified as the fruit of Fortunella Crassifolia Swingle, a plant of the genus Kumquat in the family Rutaceae, identified by Mr. Liu Yong from the School of Pharmacy, Jiangxi University of Traditional Chinese Medicine.

[0027] 1) Pre-treatment: After cleaning the fresh kumquat, crush it;

[0028] 2) reflux extraction: take by weighing 1 kg of kumquat after step 1 pulverization, add 3 times the amount of 50% ethanol aqueous solution, reflux extraction twice, the time of each extraction is 1, combine the extracts, reclaim ethanol with a rotary evaporator, Get kumquat extract extract. The yield of kumquat extract is 0.8%.

Embodiment 2

[0030] 95% Ethanol Preparation of Kumquat Extract

[0031] Kumquat: purchased from the Suichuan planting base in Jiangxi Province, identified as the fruit of Fortunella Crassifolia Swingle, a plant of the genus Kumquat in the family Rutaceae, identified by Mr. Liu Yong from the School of Pharmacy, Jiangxi University of Traditional Chinese Medicine.

[0032] 1) Pre-treatment: After cleaning the fresh kumquat, crush it;

[0033] 2) reflux extraction: take by weighing 1 kg of kumquat after step 1 pulverization, add 3 times the amount of 95% ethanol aqueous solution, reflux extraction twice, the time of each extraction is 1, combine the extracts, and reclaim ethanol with a rotary evaporator, Get kumquat extract extract. The yield of kumquat extract is 0.7%.

Embodiment 3

[0035] Content Determination of Active Components of Kumquat Extract

[0036] 1. Experimental process

[0037] Gas chromatography conditions: Agilent 19091s-433:93.92873 column, 30m×0.25mm×0.25μm. The inlet temperature was 250 °C, the total flow rate was 19.6 mL / min, the split ratio was 20:1, and the column flow rate was 1 mL / min. The interface temperature is 280°C. The heating program is 40°C for 2 minutes, rising to 250°C at 10°C / min, holding for 2 minutes, then rising to 280°C at 20°C / min, and holding for 1 minute. The carrier gas is high purity helium. The injection volume was 1 μL.

[0038] Mass spectrometry conditions: EI ionization source, electron energy at 70eV, ion source temperature at 230°C, quadrupole temperature at 150°C. The measurement method is full scan, the mass range is 35-550u, the multiplier voltage is 1482V, and the filament emission current is 34.6μA. The solvent delay time was 2.0 min.

[0039] Qualitative and quantitative: Use the NIST11.L spec...

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Abstract

The invention discloses a fortunella margarita extract as well as a preparation method and application thereof. The fortunella margarita extract is a pure natural substance extracted from fortunella margarita fruits. The fortunella margarita extract is obtained through ethanol reflux extraction, the main active ingredients and content in the fortunella margarita extract are determined by using GC-MS, and a hypertension animal model establishment test proves that the fortunella margarita extract and the active ingredients thereof can obviously reduce vasodilation pressure, regulate angiotensinII and other vasodilation factors, and besides, relieve target organ damage caused by hypertension. Cell tests prove that the HUVEC cell viability induced by the angiotensin II can be regulated. Therefore, the fortunella margarita extract has a good prevention and treatment effect on renal hypertension.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to a kumquat extract and a preparation method and application thereof. Background technique [0002] Kumquat, also known as Luofu (Oujiang Yizhi), longevity kumquat, milk orange (flower mirror), Gongsun orange, kumquat, etc., is the fruit of the Rutaceae kumquat plant Kumquat Fortunella margarita (Lour) Swingle, and the kumquat fruit is rectangular. Round or oval, golden yellow when ripe, the peel and pulp are difficult to peel off. Kumquat contains a variety of chemical components, mainly including flavonoids, volatile oils, polysaccharides and other compounds. Among them, Chen Ping and others analyzed the volatile components of kumquat fruit and identified 39 components, including 24 terpenes, 8 lipids, 1 alcohol, and 5 alkanes. Kumquat has a variety of pharmacological effects, currently found to have anti-oxidation, immune function regulation, blood lipid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A61K31/015A61K31/01A61P9/12
CPCA61K36/752A61K31/015A61K31/01A61P9/12A61K2300/00
Inventor 曾治君刘红宁张吉日木吐吉燕华李斌
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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