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A method for demolding hydrogels and biomaterials using sacrificial materials

A technology of biological materials and sacrificial materials, applied in polyether coatings, coatings, etc., can solve the problems of biological material purity interference, inability to mold stable coating, etc.

Active Publication Date: 2022-06-21
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the existing gel substrate is used as a sacrificial material in the process of biomaterial preparation, it cannot be stably coated on the mold due to its amorphous phase state. The processing method in the prior art is to use thick coating. Processing, due to the excessive immersion of biological material by thick coating, it will inevitably interfere with the purity of biological material in the mold

Method used

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  • A method for demolding hydrogels and biomaterials using sacrificial materials

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Experimental program
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Embodiment 1

[0022] Embodiment 1: a method for demolding a hydrogel and a biomaterial using a sacrificial material, comprising the following steps:

[0023] S1. Take the mole fraction of 1 part of coupling agent and 1.7 parts of deionized water, mix the coupling agent into deionized water and stir well to make the coupling agent evenly dispersed in deionized water to obtain coupling gel auxiliary agent, wherein the coupling agent The agent includes 0.7 part of 2-hydroxyethyl acrylate, 0.03 part of acrylate microgel emulsion, 0.12 part of chitosan and 0.15 part of hydroxyethyl methacrylate;

[0024] S2. Add 0.2 part of guar gum to the coupling gelling aid, heat the mixture to 35°C and stir at a speed of 60r / min for 4min;

[0025] S3. Take 12 parts of polyethylene glycol diacrylate and 36 parts of deionized water, mix polyethylene glycol diacrylate into deionized water, heat to 40° C. and stir at 120 r / min for 5 minutes to obtain polyethylene glycol diacrylate. Acrylate aqueous solution, ta...

Embodiment 2

[0033] Embodiment 2: a method for demolding a hydrogel and a biomaterial using a sacrificial material, comprising the following steps:

[0034] S1. Take the mole fraction of 1 part of coupling agent and 1.7 parts of deionized water, mix the coupling agent into deionized water and stir well to make the coupling agent evenly dispersed in deionized water to obtain coupling gel auxiliary agent, wherein the coupling agent The agent includes 0.95 part of hydroxymethyl cellulose and 0.05 part of chitosan;

[0035] S2. Add 0.2 part of guar gum to the coupling gelling aid, heat the mixture to 35°C and stir at a speed of 60r / min for 4min;

[0036] S3. Take 12 parts of polyethylene glycol diacrylate and 36 parts of deionized water, mix polyethylene glycol diacrylate into deionized water, heat to 40° C. and stir at 120 r / min for 5 minutes to obtain polyethylene glycol diacrylate. Acrylate aqueous solution, take 16 parts of dithiothreitol and 36 parts of deionized water, mix dithiothreito...

Embodiment 3

[0044] Embodiment 3: a method for demoulding hydrogel and biomaterial using traditional anti-adhesion agent, comprising the following steps:

[0045] S1. Coating synthetic silica powder on the inner wall of the mold to be used to form an insulating cushion layer of 0.01-0.028 mm;

[0046] S2. Coat 0.015mm of hydrogel on the insulating cushion and wait for it to cure;

[0047] S3. Inject the hydrogel or biological material to be formed into the mold processed in S2, and continue to stand until the cross-linking of the material is completed.

[0048] The demolded hydrogels or biomaterials in Example 1 and Example 2 were analyzed as a whole, and the interface integrity of the contact surface with the mold and the contamination degree of impurities at a depth of 0.5 mm were tested. The parameters obtained are as follows.

[0049] interface integrity Impurity contamination Example 1 99.28％ 0.015％ Example 2 99.71％ 0.072％ Example 3 98.33％ 0.285％ ...

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Abstract

The invention discloses a method for demolding hydrogels and biological materials using sacrificial materials, based on the reaction of polyethylene glycol diacrylate (PEGDA), dithiothreitol (DTT) and borax to generate a Sacrificial hydrogel that can be dissolved in a high-sugar environment and has good biocompatibility. Before using the mold, soak it in a mixed solution of 0.28mol / LPEGDA and DTT for 5 seconds, and then soak it in a 0.1mol / L borax solution 5s to quickly form a layer of gel film on the surface of the mold, and then pour in the biomaterials to be formed. After the materials are cross-linked, soak in high-sugar medium for 10-20 minutes, and the mold will fall off by itself, leaving a good shape The advantage of the present invention is that the method is convenient and fast, does not modify the material to be shaped, does not stick, and can be demoulded in a cell-friendly environment.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a method for demolding hydrogel and biological materials using sacrificial materials. Background technique [0002] When using the mold method to prepare hydrogels or other biological materials (need to inoculate cells), the material adheres to the surface of the mold during the stripping process, which is easy to tear, while the existing anti-adhesion agent is easy to adhere to the material and is not easy to wash out , which will affect subsequent biological experiments. [0003] In order to solve this problem, the general solution is to pre-coat a dense anti-stick coating on the mold to facilitate the release of the hydrogel and other biological material contents, but the dense anti-stick coating such as Teflon is coated Layers are toxic to biological materials and can cause material contamination. [0004] To solve this problem, we need a sacrificial hydrogel that is sol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L71/02C08K5/37C09D171/02C09D7/63
CPCC08J3/075C09D171/02C09D7/63C08J2371/02C08K5/37
Inventor 谢昕芳汪振星孙家明陈雳风周牧冉齐钰涵牟珊刘绍恺罗超曾宇阳侯金飞方慧敏慈海刘剑张郭王冰倩倪若飘孙谛
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV