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A kind of traditional Chinese medicine composition and its preparation method and application

A composition and technology of traditional Chinese medicine, applied in the field of traditional Chinese medicine composition and preparation thereof, can solve the problems such as inability to stop the drug at will, severe liver disease, easy recurrence, etc., and achieve the effect of good anti-HBV ability and good application prospect of anti-HBV

Active Publication Date: 2022-07-08
CAPITALBIO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interferon, nucleoside analogs and other liver disease antiviral western medicines mainly inhibit the replication of DNA. The disadvantage is that the medicine cannot be stopped casually. Long-term use will cause side effects such as flu-like, anorexia, nausea, diarrhea, depression, angina pectoris, etc.
In addition, although lamivudine and other nucleoside antiviral drugs can quickly inhibit the virus, after stopping the drug, the virus will replicate quickly, ALT will increase, and some individuals will develop severe liver disease and make the disease worse
These drugs have rapid curative effect, but are prone to relapse and have certain side effects

Method used

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  • A kind of traditional Chinese medicine composition and its preparation method and application
  • A kind of traditional Chinese medicine composition and its preparation method and application
  • A kind of traditional Chinese medicine composition and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1: Soxhlet extraction method of traditional Chinese medicine composition of the present invention

[0037] 1. Prescription

[0038] Gardenia 120, Yin Chen 180, Coptis 120, Evodia 20.

[0039] 2. Preparation method

[0040]a. Preparation of 90% ethanol: take 900 mL of absolute ethanol, add 100 mL of deionized water, and mix upside down three times.

[0041] b. Put 10 g of medicinal materials wrapped in filter paper into a Soxhlet extractor, and use 100-200 mL of 90% ethanol for extraction (material ratio 1:10-20). After the extraction, the samples were put into a drying oven to dry at 45°C and evaporated to 2 mL. Then transfer it to a 10mL brown vial, store it in a -80°C refrigerator, and freeze it for at least 6h.

[0042] c. Take out the brown vial containing the sample from the -80°C refrigerator, freeze-dry it into powder using a freeze dryer, and save it for later use.

Embodiment 2

[0043] Embodiment 2: ultrasonic preparation method of traditional Chinese medicine composition of the present invention

[0044] 1. The formula is the same as in Example 1 (120 parts of Gardenia, 180 parts of Yin Chen, 120 parts of Coptis chinensis, 20 parts of Evodia Fructus), and pulverized by a pulverizer.

[0045] 2. Add 90% ethanol solution according to the material ratio of 1:10-20, soak for 1-2h, extract by ultrasonic for 10-30min, fully stand, and concentrate the supernatant at 70°C.

[0046] 3. Take the concentrated solution, freeze it at -80°C for more than 6 hours, and then dry it in a low-temperature vacuum to obtain dry powder.

Embodiment 3

[0047] Embodiment 3: Chinese medicine composition of the present invention anti-hepatitis B virus experiment

[0048] 1. Materials and methods

[0049] (1) Traditional Chinese medicine composition of the present invention: the alcoholic extract prepared by the method in Example 1 of the present invention, take 50 mg of lyophilized powder, add 1 mL of DMSO, and prepare a concentrated solution of 50 mg / mL.

[0050] (2) Configuration of the experimental working solution of the traditional Chinese medicine composition of the present invention: according to the experimental requirements, the concentrated solution will be diluted into a working solution of 100 μg / mL with a culture medium.

[0051] (3) HepG2.2.15 cell plating: For the liver cytotoxicity assay, HepG2.2.15 cells were used. Take two cell culture flasks from the incubator, add 1 mL of trypsin to each flask, shake gently to cover the entire cell surface with trypsin, put it into the incubator to digest until the cells pa...

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Abstract

The invention relates to the technical field of traditional Chinese medicine, and discloses a traditional Chinese medicine composition and a preparation method and application thereof. The traditional Chinese medicine composition of the present invention is the extract of Yinchen, Gardenia, Coptis chinensis and Evodia Fructus. The traditional Chinese medicine composition with obvious anti-hepatitis B virus effect is prepared by compounding Gardenia, Yinchen, Coptidis and Evodia, and has obvious inhibitory effect on the secretion of HBsAg and HBeAg antigens. The toxicity to hepatocytes makes the whole formula have no obvious inhibitory effect on the activity of hepatocytes, and at the same time, it has a significant anti-hepatitis B virus effect compared with each single drug. In addition, the n-butanol-extracted active site of the traditional Chinese medicine composition of the present invention also has no obvious HepG2.2.15 hepatic cytotoxicity, and has obvious inhibitory effect on the secretion of HBsAg and HBeAg antigens.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a traditional Chinese medicine composition and a preparation method and application thereof. Background technique [0002] The liver is the largest solid organ in the abdominal cavity and is responsible for the important physiological functions of the human body. Its main function is to regulate material metabolism and energy metabolism in the whole body, and it is also an important immune organ. Liver irritation or infection such as infectious diseases, immunodeficiency diseases, and chemical poisons can cause liver damage. In my country, viral hepatitis, especially chronic hepatitis B, is the most common cause of liver damage and eventually cirrhosis. Anti-HBV therapy is the key to preventing further progression of liver damage. [0003] Antiviral therapy is the fundamental treatment method for chronic hepatitis B. For hepatitis B patients w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/754A61P31/20A61P1/16
CPCA61K36/282A61K36/744A61K36/718A61K36/754A61P31/20A61P1/16A61K2236/333A61K2236/55A61K2300/00Y02A50/30
Inventor 郭弘妍王辉郝艳同邢韶芳程京
Owner CAPITALBIO CORP
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