A kind of preparation method of high aroma tobacco extract
A technology for tobacco extract and aroma, applied in the field of preparation of high-aroma tobacco extract, can solve problems such as adverse effects on environment and production cost, and achieve the effects of saving solvent cost, good quality and cost reduction
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Embodiment 1
[0018] Dry the waste tobacco powder at 40°C until the moisture is less than 2.0%, crush it through a 40-mesh sieve and mix well, weigh 0.1g hemicellulase into a beaker, dissolve it with 1.2mL citric acid buffer (pH=4.5) , add 200mL of deionized water, 23mL of citric acid buffer and 50g of waste tobacco powder crushed through a 40-mesh sieve, mix well, put it in a water bath at 50°C and stir for 1.5h for enzymolysis, and centrifuge for 10 Minutes, centrifuged to obtain primary enzymatic hydrolysis solution and tobacco powder A after enzymolysis, add hemicellulase 0.03g, deionized water 130mL and citric acid buffer 6mL with pH value 4.5 to the primary enzymolysis solution, and then take another Put 50 grams of waste tobacco powder that has been crushed through a 40-mesh sieve into the primary enzymatic hydrolysis solution, mix well, stir the enzymatic hydrolysis in a water bath at 50°C for 1.5 hours, centrifuge for 10 minutes after the enzymatic hydrolysis, and obtain The second...
Embodiment 2
[0023] Example 2: Dried the waste tobacco powder at 40°C until the moisture content was less than 2.0%, crushed it through a 40-mesh sieve and mixed it evenly, weighed 0.05g of hemicellulase and put it into a beaker. 4.5) After dissolving, add 220mL of deionized water, 30mL of citric acid buffer and 50g of waste tobacco powder that has been crushed and passed through a 40-mesh sieve. Centrifuge for 10 minutes after the end, and centrifuge to obtain the primary enzymolysis solution and the tobacco dust A after enzymolysis. Add 0.04 g of hemicellulase, 120 mL of deionized water and 6 mL of citric acid buffer solution with a pH value of 4.5 to the primary enzymolysis solution , and then take another 50 grams of waste tobacco dust that has been crushed through a 40-mesh sieve and put it into the primary enzymatic hydrolysis solution. Separate the secondary enzymatic hydrolysis solution and the tobacco powder B after enzymatic hydrolysis, and further supplement hemicellulase, deion...
Embodiment 3
[0026] Example 3: Dry the waste tobacco powder at 45°C until the moisture content is less than 2.0%, pulverize it, pass through a 40-mesh sieve and mix evenly, weigh 0.15g of hemicellulase into a beaker, and use 3mL of citric acid buffer (pH=4.5 ) after dissolving, add 250mL of deionized water, 25mL of citric acid buffer and 50g of waste tobacco powder crushed through a 40-mesh sieve. Centrifuge for 10 minutes, centrifuge to obtain the primary enzymatic hydrolysis solution and tobacco dust A after enzymatic hydrolysis, add hemicellulase 0.05g, deionized water 140mL and citric acid buffer solution 7mL with a pH value of 4.5 to the primary enzymolysis solution, and then Take another 50 grams of waste tobacco dust that has been crushed through a 40-mesh sieve and put it into the primary enzymatic hydrolysis solution. After fully mixing, stir the enzymatic hydrolysis in a water bath at 50°C for 1 hour. After the enzymatic hydrolysis, centrifuge for 10 minutes to obtain Secondary e...
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