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A kind of polypeptide, preparation method and medical use thereof

A technology of drying and Kochia seeds, applied in the field of peptides, can solve the problems of peptide separation and less research on activity.

Active Publication Date: 2022-06-17
阿南达
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on the isolation and activity of polypeptides in Kochia chinensis

Method used

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  • A kind of polypeptide, preparation method and medical use thereof
  • A kind of polypeptide, preparation method and medical use thereof
  • A kind of polypeptide, preparation method and medical use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Experimental materials

[0021] Grind the dried Kochia seeds through a 60-mesh sieve and set aside.

[0022] The purified water was bottled by Wahaha; the alkaline protease was purchased from Novozymes.

[0023] 2. Experimental method

[0024] Step S1: Alkaline extraction and acid precipitation to extract total protein

[0025] 1 kg of Kochia kohlrabi was extracted with 10 L of absolute ethanol under reflux for 3 hours, and the residual solvent was evaporated by filtration. Add 10L of purified water, adjust the pH to 11.0 with 10% sodium hydroxide solution, and stir and extract at room temperature for 6h. The extract was first filtered to collect the filtrate, then centrifuged at 4000r / min for 20min, took the supernatant, adjusted the pH to 4.8 with 10% hydrochloric acid solution, left standing for 2h, centrifuged at 4000r / min for 20min, collected the precipitate, and freeze-dried to obtain Kochia total protein.

[0026] Step S2: protease enzymatic hydrolysis

...

Embodiment 2

[0032] 1. Experimental materials

[0033] Kochia kochia enzymolysis polypeptide was prepared according to the method of Example 1.

[0034] New Zealand male rabbits weighed (2±0.2) kg, and were used for one week after adaptive feeding.

[0035] 2. Experimental method

[0036] The in vitro anti-platelet aggregation activity was determined by the Born turbidimetric method. For the specific operation method, please refer to the literature (Tang Quan et al., Design, Synthesis and Anti-platelet Aggregation Research of Indobufenamide Compounds, 2020):

[0037] The rabbits were locally anesthetized with 1% procaine hydrochloride, and the carotid artery was cannulated to collect blood into 3.8% sodium citrate solution (the volume ratio of blood to sodium citrate solution was 9:1), Centrifuge at 1000 r / min for 15 min to obtain platelet rich plasma (PRP). Different concentrations of Kochia kohlrabi enzymolyzed polypeptide solutions were prepared with physiological saline as solvent. ...

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PUM

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Abstract

The invention discloses a polypeptide, a preparation method and its medical use. Kochia officinalis is the dry ripe fruit of Kochia scopariae of Chenopodiaceae. The invention provides an alkaline protease hydrolyzed polypeptide of Kochia chinensis with a molecular weight between 3000u and 5000u. Activity tests show that the enzymolyzed polypeptide has obvious anti-platelet aggregation activity, and this activity increases with the enzymatic hydrolysis of Kochia chinensis. Enhanced by increasing the concentration of the polypeptide, it can be used to develop anti-platelet aggregation drugs.

Description

technical field [0001] The invention belongs to the field of polypeptides, and in particular relates to a polypeptide, a preparation method and its medical use. Background technique [0002] Cardiovascular and cerebrovascular diseases refer to ischemic or hemorrhagic diseases of the heart, brain and systemic tissues caused by hyperlipidemia, hypertension, blood viscosity, atherosclerosis, etc., which pose a serious threat to human health. In recent years, with the aggravation of aging and the accelerated pace of life in my country, the incidence of cardiovascular and cerebrovascular diseases has been increasing year by year, and it has become the number one killer of residents. Molecular biology studies have shown that the pathogenesis of most cardiovascular and cerebrovascular diseases is related to arterial and venous thrombosis, and platelet aggregation is one of the main factors leading to thrombosis (Chen Xiaoman et al, Design of Paeonol Ester Compounds and Research on ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C07K1/14C07K1/34C12P21/06A61K38/16A61K38/01A61P7/02
CPCC07K14/415C12P21/06A61P7/02A61K38/00
Inventor 郭守山其他发明人请求不公开姓名
Owner 阿南达
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