Globulin extraction method based on nuclear magnetic resonance detection

A technology of nuclear magnetic resonance and extraction method, which is applied in the direction of analysis by nuclear magnetic resonance, preparation of test samples, material analysis through resonance, etc. It can solve problems such as complex extraction process, achieve reasonable process, ingenious extraction method, and improved purity Effect

Inactive Publication Date: 2021-03-23
安徽标王食品有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the shortcomings of complex extraction process in the prior art, and propose a method for extracting globulin based on nuclear magnetic resonance detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021]A globulin extraction method based on nuclear magnetic resonance detection, including the following steps,

[0022]S1, 12G alkaline protease was added to 400 mL of phosphate buffer, placed in an ultrasonic oscillator, oscillating 30 min at a 100 W power ultrasound, centrifuge with the hemister centrifuge at 6000 r / min at a rotational speed of 25 min, separated removal The supernatant, obtained immobilized protease, spare;

[0023]S2, weigh 600G walnuts into the warm water of 60 ° C for 1 h, put the walnuts into the pulverizer and fully pulverized, and then put it into the reaction tank, add centrifuge, 3000R / min. The mixed solution A was centrifuged for 30 minutes, and the supernatant A was collected;

[0024]S3, the above supernatant A is moved into the enzyme solution, adding the total mass of the total mass of the material to 1% of the spare immobilization protease, and placed in the inclumpt in the inclumpted film, raised to 40 ° C, after the enzymatic solution for 6 h, A resil...

Embodiment 2

[0028]A globulin extraction method based on nuclear magnetic resonance detection, including the following steps,

[0029]S1, 17 g of alkaline protease was added to 450 ml of phosphate buffer, placed in an ultrasonic oscillator, oscillating 33min at 150 W power, and centrifuge with a hemiscutant at 7000 r / min for 30 min, separated removal The supernatant, obtained immobilized protease, spare;

[0030]S2, weigh 650G walnuts into warm water in 65 ° C for 2 h, put the walnuts into the pulverizer, stir it, and then put it into the reaction can, add centrifuge, 4000R / min under conditions. The mixed solution A was centrifuged for 40 minutes, and the supernatant A was collected;

[0031]S3, the above supernatant A is moved into the enzyme solution, and the total mass of the total mass of the material is added, and the tank is added to the tin of the tank, and then the temperature is placed to 40 ° C, and after the enzymatic solution is 6h, A resiling magnetic field is applied around the enzymati...

Embodiment 3

[0035]A globulin extraction method based on nuclear magnetic resonance detection, including the following steps,

[0036]S1, 20G alkaline protease was added to 500 ml of phosphate buffer, placed in an ultrasonic oscillator, oscillating 33min at 200W power, and centrifuge with the hemister centrifuge at 7000 r / min for 30 min, separated removal The supernatant, obtained immobilized protease, spare;

[0037]S2, weighing 700 g walnuts into the warm water of 70 ° C for 2 h, put the walnuts into the pulverizer, stirred into the pulverizer, and then put it in the reaction tank, add centrifuge, 4000R / min under conditions The mixed solution A was centrifuged for 40 minutes, and the supernatant A was collected;

[0038]S3, the above supernatant A is moved into the enzyme solution, and the total mass of the total mass of the material is added, and the tank is added to the tin of the tank with a plastic wrap, and then the temperature is increased to 50 ° C, after the enzymatic solution of 8 h, A res...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a globulin extraction method based on nuclear magnetic resonance detection. The method comprises the following steps: weighing 15-20g of alkaline protease, adding into 400-500mL of phosphoric acid buffer solution, putting into an ultrasonic oscillator, ultrasonically oscillating at the power of 100-200W for 30-33min, centrifuging the reaction solution by using a horizontalcentrifuge at the rotating speed of 6000-7000r / min for 25-30min, and separating to remove supernatant to obtain immobilized protease for later use; weighing 600-700 g of walnut kernels and putting into warm water at 60-70 DEG C to be soaked for 1-2 h, putting the walnut kernels into a pulverizer to be fully pulverized, putting the walnut kernels into a reaction tank after being pulverized, addinginto a centrifugal machine to be subjected to centrifugal treatment, centrifuging the mixed solution A for 30-40 min under the condition of 3000-4000 r / min, and collecting a supernatant A.

Description

Technical field[0001]The present invention relates to the field of walnut protein peptide processing, and more particularly to a globulin extraction method based on nuclear magnetic resonance detection.Background technique[0002]Walnuts, also known as walnuts, porridge, is a walnut plant. With a flat peach, cashews, hazelnuts and called the world famous "four dried fruits". Walnuts contain rich nutrients, 15 to 20 grams per bicker, more fat, 10 grams of carbohydrate, and contain a variety of trace elements and minerals such as calcium, phosphorus, iron, and carotene, nuclear Variety of vitamins such as lumens. It is beneficial to the human body. It is one of the nut foods that are popular with the people. Walnuts are hard and need to be worried. Walnuts contains a globulin, while the existing walnuts extracts the process complex, high cost, and cannot perform nuclear magnetic resonance detection of extracted globulins. In this respect, the present invention proposes a nuclear magneti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/34G01N24/08
CPCG01N1/286G01N1/34G01N24/08G01N24/087G01N2001/2866
Inventor 尹琪王营王夺标周军刘梧林戚化学陈少生
Owner 安徽标王食品有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap