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Preparation method of bacillus thuringiensis and tea saponin compound microcapsule preparation

A technology of Bacillus aureus and spore thuringiensis, which is applied in the fields of botanical equipment and methods, fungicides, chemicals for biological control, etc. Uniformity and other problems to achieve the effect of improving insecticidal and disease prevention, reducing the amount of use, and overcoming the slow effect

Inactive Publication Date: 2021-03-30
PUTIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are more than 120 kinds of Bt preparations, accounting for more than 90% of microbial pesticides. The commonly used formulations include wettable powders, oils, oil emulsions, suspensions, and sustained release agents. Among them, wettable powders do not contain organic solvents and are effective. With the advantages of high content of ingredients and good control effect, it accounts for a large proportion in the pesticide market, but the suspension rate of wettable powder is low, which can easily lead to blockage of the nozzle of the sprayer, and the uneven concentration of the liquid medicine is easy to cause phytotoxicity; the emulsifiable concentrate is One of the main dosage forms of pesticide preparations in various countries. It has the characteristics of simple preparation process and good control effect. However, it consumes a lot of organic solvents such as xylene and toluene during production, which will pollute the environment and needs further improvement.

Method used

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  • Preparation method of bacillus thuringiensis and tea saponin compound microcapsule preparation
  • Preparation method of bacillus thuringiensis and tea saponin compound microcapsule preparation
  • Preparation method of bacillus thuringiensis and tea saponin compound microcapsule preparation

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Experimental program
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Effect test

Embodiment 1

[0028] (1) Bacillus thuringiensis HD109 strain was inserted into an optimized medium containing 2 g / L sucrose, 20 g / L peptone and 2.5% colloidal chitin at an inoculum amount of 1.572%, and the initial pH of the optimized medium was 8 , under the condition of 30 ℃, 200rpm, vibrating for 48h to obtain the optimized Bacillus thuringiensis HD109 strain (OD 600 The value is 3.8645, and the enzyme activity value is as high as 2.7961U / mL);

[0029] (2) The above-mentioned optimized Bacillus thuringiensis HD109 strain was inserted into BPY medium with an inoculum of 1%, and under the conditions of 30 ° C and 200 pm, the activated strain was shaken for 24 hours, and then the activated Bacillus thuringiensis spore Bacillus HD109 strain was transferred to another BPY medium at 3% of the inoculum, and under the conditions of 30 ° C and 220 rpm, after shaking culture for 50-60 h, centrifuged, and discarded the supernatant to obtain Bacillus thuringiensis HD109 strain somatic mixture;

[...

Embodiment 2

[0035] 1. Determination of the storage stability index of the final product of microencapsulated insecticide

[0036] The Bacillus thuringiensis microcapsule preparation (Bt microcapsules) prepared in Example 1 was diluted 50 times and the particle size of the microcapsules was measured under a microscope, and its shape was observed, and the spore decomposition rate was measured after the ultraviolet lamp irradiation (marked). Plate) and entrapment rate (using a hemocytometer to count microencapsulated Bacillus thuringiensis and non-microencapsulated Bacillus thuringiensis under a microscope) to determine whether the quality of microencapsulated pesticides meets the standard, and the microencapsulated Bacillus thuringiensis is counted under a microscope. The storage and stability of the microcapsule insecticides were observed after the capsule insecticides were stored at -15°C, 0°C, 25°C, and 50°C for 14 days, respectively.

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Abstract

The invention discloses a preparation method of a bacillus thuringiensis and tea saponin compound microcapsule preparation. According to the invention, an optimized bacillus thuringiensis HD109 strainis compounded with tea saponin, and then a microcapsule insecticide is prepared from sodium alginate, gelatin and calcium chloride, so that the dosage of the insecticide is reduced, the problem thatbacillus thuringiensis is intolerant to ultraviolet irradiation is solved, the insecticidal and disease-preventing effects are improved, and the pesticide effect is more durable.

Description

technical field [0001] The invention belongs to the technical field of microcapsule insecticide preparation, in particular to a preparation method of a Bacillus thuringiensis and tea saponin compound microcapsule preparation. Background technique [0002] Bacillus thuringiensis (Bt) is a gram-positive bacterium that produces insecticidal crystal proteins, parasporal crystals, during the formation of its spores, which makes Bt have special insecticidal activity and is widely used in sanitary Biological control of pests, forestry, agriculture. However, Bt preparations still have defects such as narrow insecticidal spectrum, unstable control effect, and slow insecticidal speed. Therefore, in order to improve the control effect of Bt preparations, the research on its synergy has become one of the world's research hotspots. [0003] Tea saponin is a plant-derived compound with biological activities such as killing acaricide, bactericidal, and insecticidal. Research at home and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/23A01N43/16A01N25/28A01P3/00A01P7/02A01P7/04
CPCA01N25/28A01N43/16
Inventor 傅丽君杨磊
Owner PUTIAN UNIV
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