Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting iridoid glycoside in gentianella turkestanorum by UPLC-MS/MS

A technology for iridoid glycosides and gentiopicrosides, applied in the field of detection and analysis, can solve problems such as research reports on the pharmacokinetic characteristics of Xinjiang pseudogentian iridoid glycosides that have not yet been seen, and achieve reproducibility Good, high operation requirements, good separation effect

Pending Publication Date: 2021-03-30
THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing reports are limited to the content determination of the chemical components of Xinjiang pseudogentian and a few pharmacological studies, but there is no research on the pharmacokinetic characteristics of Xinjiang pseudogentian iridoid glycosides using UPLC-MS / MS technology to report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting iridoid glycoside in gentianella turkestanorum by UPLC-MS/MS
  • Method for detecting iridoid glycoside in gentianella turkestanorum by UPLC-MS/MS
  • Method for detecting iridoid glycoside in gentianella turkestanorum by UPLC-MS/MS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]Take three kinds of iridoid glycoside reference substances, place them in a 10mL measuring bottle, dissolve them with methanol and dilute to the mark, and shake well to obtain gentiopicroside, swertiamarin and Swertiin standard solution. After preparing the sample solution of Xinjiang pseudogentiana, carry out pretreatment, first vortex for 1min, then add 300 μL of acetonitrile, absorb the supernatant after centrifugation, blow dry with nitrogen, then add 100 μL of methanol water (the volume ratio of methanol to water is 1: 1) Reconstitute and vortex for 1 min, process at a centrifugation speed of 12000 r / min for 10 min, absorb the supernatant and pass through a 0.22 μm organic filter membrane, and finally perform UPLC-MS / MS analysis.

[0039] Mass spectrometry conditions are positive ion detection; ion source is electrospray ion source (ESI source); scanning method is selective reaction monitoring; capillary voltage: 3500V; cone voltage: 45V; extraction cone voltage: 3V...

Embodiment 2

[0042] Take three kinds of iridoid glycoside reference substances, place them in a 10mL measuring bottle, dissolve them with methanol and dilute to the mark, and shake well to obtain gentiopicroside, swertiamarin and Swertiin standard solution. After preparing the sample solution of Xinjiang pseudogentiana, carry out pretreatment, first vortex 2min, add 200 μ L of acetonitrile, absorb the supernatant after centrifugation, blow dry with nitrogen, then add 50 μ L of methanol water (the volume ratio of methanol and water is 1: 1) Reconstitute and vortex for 2 minutes, process at a centrifugal speed of 10000r / min for 8 minutes, absorb the supernatant, pass through a 0.22 μm organic filter membrane, and finally perform UPLC-MS / MS analysis.

[0043] Mass spectrometry conditions are positive ion detection; ion source is electrospray ion source (ESI source); scanning method is selective reaction monitoring; capillary voltage: 3500V; cone voltage: 45V; extraction cone voltage: 3V; RF l...

Embodiment 3

[0046] Take three kinds of iridoid glycoside reference substances, place them in a 10mL measuring bottle, dissolve them with methanol and dilute to the mark, and shake well to obtain gentiopicroside, swertiamarin and Swertiin standard solution. After preparing the sample solution of Xinjiang pseudogentiana, carry out pretreatment, first vortex 3min, add 100 μ L acetonitrile, absorb the supernatant after centrifugation, blow dry with nitrogen, then add 80 μ L methanol water (the volume ratio of methanol and water is 1: 2) Reconstitute and vortex for 3 minutes, process at a centrifugation speed of 8000r / min for 9 minutes, absorb the supernatant, pass through a 0.22 μm organic filter membrane, and finally perform UPLC-MS / MS analysis.

[0047] Mass spectrometry conditions are positive ion detection; ion source is electrospray ion source (ESI source); scanning method is selective reaction monitoring; capillary voltage: 3500V; cone voltage: 45V; extraction cone voltage: 3V; RF lens ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for detecting iridoid glycoside in gentianella turkestanorum by the UPLC-MS / MS. The method comprises the following steps of: (1) respectively preparing gentiopicroside, swertiamarin and swertiamarin into standard solutions with the concentration of 0.25-0.5 mg / mL for later use; and (2) preparing a sample solution of gentianella turkestanorum, then carrying out pretreatment on the sample solution, and detecting iridoid glycoside in the gentianella turkestanorum by using the UPLC-MS / MS method. The UPLC-MS / MS technology is selected as an active component detectionand analysis method to detect iridoid glycoside in the gentianella turkestanorum; and the method has the advantages of being rapid, sensitive, stable and easy to reproduce.

Description

technical field [0001] The invention relates to the technical field of detection and analysis, and more specifically relates to a method for detecting iridoid glycosides in Xinjiang pseudogentiana by UPLC-MS / MS. Background technique [0002] Gentianella turkestanerum (Gand.) Holub is a plant belonging to Gentianaceae (Gentiananceae) and is one of the dominant medicinal resources in Xinjiang. Xinjiang false gentian is a commonly used medicinal material in Uyghur, Mongolian and Tibetan medicine. It has the functions of clearing heat and detoxifying, dampness and swelling. In terms of chemical composition, some scholars have done chemical research on the plants of the genus Pseudogentian, and the main compounds isolated are iridoid glycosides, which are monoterpene compounds with the basic skeleton of cyclopentadienopyran ring, and other There are two sesquiterpenoids, triterpenoids and so on. In the early stage, the inventor determined the content of 5 active ingredients in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/72G01N30/04G01N30/06G01N30/14G01N30/86G01N30/88
CPCG01N30/02G01N30/72G01N30/04G01N30/06G01N30/14G01N30/8634G01N30/88G01N2030/042G01N2030/146G01N2030/062G01N2030/884
Inventor 杨建华胡君萍居博伟
Owner THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com