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Establishment method of fingerprint spectrum of blood-enriching motherwort pills and quality detection method

A technology for fingerprint and method establishment, applied in the field of traditional Chinese medicine analysis and detection, can solve the problems of single quality control standard, difficult to accurately assess the quality of tonifying blood and Yimu preparations, weak and other problems, achieve a good linear relationship, ensure quality and clinical efficacy, peak good effect

Pending Publication Date: 2022-01-21
ZHUZHOU QIANJIN PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems in the prior art that the quality detection method of the Buxue Yimu preparation is not comprehensive enough, and the quality control standard is single and relatively weak, which makes it difficult to accurately evaluate the quality of the Buxue Yimu preparation in the market. Establishment of fingerprints of Buxue Yimu Pills and its quality detection method

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  • Establishment method of fingerprint spectrum of blood-enriching motherwort pills and quality detection method
  • Establishment method of fingerprint spectrum of blood-enriching motherwort pills and quality detection method
  • Establishment method of fingerprint spectrum of blood-enriching motherwort pills and quality detection method

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Experimental program
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Effect test

Embodiment 1

[0045] The optimization of embodiment 1 chromatographic conditions

[0046] In the method of establishing fingerprints, because the chemical components in different Chinese medicinal materials vary widely, the elution conditions of fingerprints of different medicinal materials and preparations have no reference value to each other, and small changes in the mobile phase ratio will affect the fingerprints. It has a great impact, such as affecting the resolution and peak shape of characteristic peaks. In order to make the characteristic peaks in the fingerprints have better resolution and peak shape, the selection of gradient elution conditions is very important. In this embodiment, the mobile phase and detection wavelength are mainly investigated and optimized.

[0047] 1. Selection of mobile phase

[0048] Take an appropriate amount of Buxue Yimu pills, pulverize them, accurately weigh about 3.0g into a 50mL measuring bottle, add 10mL of water and sonicate for 10min, then add ...

Embodiment 2

[0055] The optimization of embodiment 2 Buxue Yimu pills test solution preparation method

[0056] In the establishment method of the fingerprint, in order to make the operation more convenient and time-consuming, and to make each characteristic peak in the fingerprint have better resolution and peak shape, and to reasonably control the detection time of the fingerprint, It is very important to investigate and optimize the preparation method of the test product. In this embodiment, the extraction sample amount, extraction solvent and extraction time are mainly investigated and optimized.

[0057] This optimization is detected under the chromatographic conditions determined in Example 1.

[0058] 1. Investigation on the amount of extracted samples

[0059] Take an appropriate amount of Buxue Yimu Pills, crush them, and accurately weigh three different samples of 3g, 1g, and 0.5g into a 50mL measuring bottle, add 10mL of water and sonicate for 10min, then add 40mL of acetonitri...

Embodiment 3

[0073] Embodiment 3 methodological investigation

[0074] Preparation of reference solution:

[0075] Precisely weigh 7.96mg of motherwort, 13.03mg of calycosin glucoside, 3.53mg of rutin, 4.88mg of ferulic acid, and 2.71mg of ligustilide reference substance, place them in 6 measuring bottles, add methanol to prepare the mass concentration 796.0, 1303.0, 353.0, 488.0, 2710.0 μg / mL single reference substance stock solution in sequence. Another appropriate amount of the above-mentioned 5 kinds of reference substance stock solutions was taken respectively, and added to a 10mL measuring bottle, and the mass concentrations of leonurine, calycosin glucoside, rutin, ferulic acid, and ligustilide were respectively 7.96, 5.47, 35.30, 58.56, 100.27μg / mL mixed reference solution.

[0076] Preparation of the test solution:

[0077] Determining the preferred test solution preparation method according to Example 2 to prepare the Buxue Yimu Pill test solution.

[0078] 1. System adaptabili...

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Abstract

The invention discloses an establishment method of a fingerprint spectrum of a blood-enriching motherwort pills and a quality detection method. The establishment method of the fingerprint spectrum of the blood-enriching motherwort pills comprises the following steps: preparing a reference solution and a test solution, establishing chromatographic conditions and performing HPLC (High Performance Liquid Chromatography) detection. The fingerprint spectrum established by the invention can comprehensively reflect chemical components in the blood-enriching motherwort pill, has the advantages of good chromatographic peak separation, stable base line, good peak pattern, good repeatability, good linear relationship, good precision, good stability and good recovery rate, and can accurately detect the content of index components in the blood-enriching motherwort pills; the obtained control fingerprint spectrum can objectively evaluate the quality of the blood-enriching motherwort pill sample, and provides a research standard for quality control of the blood-enriching motherwort pill, so as to ensure the quality and clinical curative effect of the product.

Description

technical field [0001] The invention relates to the field of analysis and detection of traditional Chinese medicines, in particular to a method for establishing fingerprints of Buxue Yimu pills and quality detection. Background technique [0002] Pelvic spasm is often accompanied by bleeding, mood swings, fatigue and other symptoms, which can easily lead to secondary diseases such as anemia and ischemia. Clinically, non-steroidal anti-inflammatory drugs (NSAIDs) and cyclooxygenase-2 (COX-2) inhibitors, such as ibuprofen and naproxen, are commonly used to treat gynecological inflammation. Although these drugs can relieve dysmenorrhea, they are easy to Cause nausea, peptic ulcer, diarrhea and other serious adverse reactions. Buxue Yimu Pill is a Chinese patent medicine developed and produced by Hunan Zhuzhou Qianjin Pharmaceutical Co., Ltd. The compound is composed of angelica, motherwort, astragalus, donkey-hide gelatin and tangerine peel, among which "angelia- Motherwort" ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8686G01N30/8631G01N30/8637
Inventor 龚云吴梦瑶邹亮王安齐张艳李维
Owner ZHUZHOU QIANJIN PHARMA
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